PMID- 28359196 OWN - NLM STAT- MEDLINE DCOM- 20170919 LR - 20170919 IS - 1793-6853 (Electronic) IS - 0192-415X (Linking) VI - 45 IP - 3 DP - 2017 TI - Prunella vulgaris Attenuates Diabetic Renal Injury by Suppressing Glomerular Fibrosis and Inflammation. PG - 475-495 LID - 10.1142/S0192415X1750029X [doi] AB - Diabetic nephropathy is both the most common complication and the leading cause of mortality associated with diabetes. Prunella vulgaris, a well-known traditional medicinal plant, is used for the cure of abscess, scrofula, hypertension and urinary diseases. This study confirmed whether an aqueous extract of Prunella vulgaris (APV) suppresses renal inflammation and fibrosis. In human mesangial cell (HMC), pretreatment of APV attenuated 25[Formula: see text]mM HG-induced suppressed TGF-[Formula: see text] and Smad-2/4 expression; it increased the expression level of Smad-7. Connective tissue growth factor (CTGF) and collagen IV, fibrosis biomarkers, were significantly decreased by APV. APV suppressed inflammatory factors such as intracellular cell adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein-1 (MCP-1). APV inhibited activation and translocation of nuclear factor kappa-B (NF-[Formula: see text]B) in HG-stimulated HMCs. Moreover, APV significantly improved HG-induced ROS in a dose-dependent manner. In diabetic rat models, APV significantly decreased blood glucose, blood urea nitrogen (BUN) and ameliorated plasma creatinine (PCr). APV reduced the PAS positivity staining intensity and basement membrane thickening in glomeruli of diabetic rats. Fibrosis related proteins such as collagen IV and TGF-[Formula: see text]1 were also inhibited by APV. These results suggest that APV has a significant protective effect against diabetic renal dysfunction including inflammation and fibrosis through disruption of the TGF-[Formula: see text]/Smad signaling. Therefore, APV may be useful in potential therapies that target glomerulonephritis and glomerulosclerosis, which lead to diabetic nephropathy. FAU - Namgung, Seung AU - Namgung S AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Yoon, Jung Joo AU - Yoon JJ AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Yoon, Chi-Su AU - Yoon CS AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - double dagger College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Han, Byung Hyuk AU - Han BH AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Choi, Eun Sik AU - Choi ES AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Oh, Hyuncheol AU - Oh H AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - double dagger College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Kim, Youn-Chul AU - Kim YC AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - double dagger College of Pharmacy, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Lee, Yun Jung AU - Lee YJ AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Kang, Dae Gill AU - Kang DG AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. FAU - Lee, Ho Sub AU - Lee HS AD - * College of Oriental Medicine and Professional, Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. AD - dagger Hanbang Body-fluid Research Center, Wonkwang University, Iksan, Jeonbuk 54538, Republic of Korea. LA - eng PT - Journal Article DEP - 20170330 PL - Singapore TA - Am J Chin Med JT - The American journal of Chinese medicine JID - 7901431 RN - 0 (Ccl2 protein, rat) RN - 0 (Chemokine CCL2) RN - 0 (Plant Extracts) RN - 0 (Reactive Oxygen Species) RN - 0 (Transforming Growth Factor beta1) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) RN - 139568-91-5 (Connective Tissue Growth Factor) RN - 9007-34-5 (Collagen) SB - IM MH - Animals MH - Cells, Cultured MH - Chemokine CCL2/metabolism MH - Collagen/metabolism MH - Connective Tissue Growth Factor/metabolism MH - Diabetic Nephropathies/*drug therapy/metabolism/pathology MH - Disease Models, Animal MH - Dose-Response Relationship, Drug MH - Fibrosis/drug therapy/metabolism MH - Glomerulonephritis/*drug therapy MH - Humans MH - Inflammation/*drug therapy MH - Intercellular Adhesion Molecule-1/metabolism MH - Kidney Glomerulus/*pathology MH - Male MH - *Molecular Targeted Therapy MH - *Phytotherapy MH - Plant Extracts/*isolation & purification/pharmacology/*therapeutic use MH - Prunella/*chemistry MH - Rats, Sprague-Dawley MH - Reactive Oxygen Species/metabolism MH - Transforming Growth Factor beta1/metabolism OTO - NOTNLM OT - Diabetic Nephropathy OT - Glomerular Fibrosis OT - Mesangial Cells OT - Prunella vulgaris OT - Streptozotocin EDAT- 2017/04/01 06:00 MHDA- 2017/09/20 06:00 CRDT- 2017/04/01 06:00 PHST- 2017/04/01 06:00 [pubmed] PHST- 2017/09/20 06:00 [medline] PHST- 2017/04/01 06:00 [entrez] AID - 10.1142/S0192415X1750029X [doi] PST - ppublish SO - Am J Chin Med. 2017;45(3):475-495. doi: 10.1142/S0192415X1750029X. Epub 2017 Mar 30.