PMID- 28359950
OWN - NLM
STAT- MEDLINE
DCOM- 20180206
LR  - 20180907
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 350
DP  - 2017 May 14
TI  - Whole transcriptome expression of trigeminal ganglia compared to dorsal root 
      ganglia in Rattus Norvegicus.
PG  - 169-179
LID - S0306-4522(17)30193-8 [pii]
LID - 10.1016/j.neuroscience.2017.03.027 [doi]
AB  - The trigeminal ganglia (TG) subserving the head and the dorsal root ganglia (DRG) 
      subserving the rest of the body are homologous handling sensory neurons. 
      Differences exist, as a number of signaling substances cause headache but no pain 
      in the rest of the body. To date, very few genes involved in this difference have 
      been identified. We aim to reveal basal gene expression levels in TG and DRG and 
      detect genes that are differentially expressed (DE) between TG and DRG. 
      RNA-Sequencing from six naive rats describes the whole transcriptome expression 
      profiles of TG and DRG. Differential expression analysis was followed by pathway 
      analysis to identify DE processes between TG and DRG. In total, 64 genes had 
      higher and 55 genes had lower expressed levels in TG than DRG. Higher expressed 
      genes, including S1pr5 and Gjc2, have been related to phospholipase activity. The 
      lower expressed genes, including several Hox genes and Slc5a7, have been related 
      to tyrosine and phenylalanine metabolism. Tissue-specific expression was 
      identified for Gabra6 and Gabrd in TG, and for several Hox genes in DRG. 
      Furthermore, genes that were known to be associated with headache/migraine were 
      mostly moderately to highly expressed in one or both tissues. We present a 
      comprehensive overview of the expression profiles of whole tissue comparison of 
      TG and DRG. Further, we showed DE genes/pathways between TG and DRG, including 
      several known migraine-associated genes. This study provides a basis for further 
      pain-related studies using TG and DRG in rats.
CI  - Copyright (c) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Kogelman, Lisette Johanna Antonia
AU  - Kogelman LJA
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark.
FAU - Christensen, Rikke Elgaard
AU  - Christensen RE
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark.
FAU - Pedersen, Sara Hougaard
AU  - Pedersen SH
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark.
FAU - Bertalan, Marcelo
AU  - Bertalan M
AD  - Institute of Biological Psychiatry, Mental Health Center Sct. Hans, University of 
      Copenhagen, Copenhagen, Roskilde DK 4000, Denmark.
FAU - Hansen, Thomas Folkmann
AU  - Hansen TF
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark.
FAU - Jansen-Olesen, Inger
AU  - Jansen-Olesen I
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark.
FAU - Olesen, Jes
AU  - Olesen J
AD  - Danish Headache Center, Department of Neurology, Glostrup Research Institute, 
      Rigshospitalet Glostrup, Glostrup, Denmark and Faculty of Health and Medical 
      Sciences, University of Copenhagen, Denmark. Electronic address: 
      https://www.rigshospitalet.dk/english/departments/neuroscience-centre/danish-headache-center.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170327
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
SB  - IM
MH  - Animals
MH  - Ganglia, Spinal/*metabolism
MH  - Gene Expression/*physiology
MH  - Male
MH  - Rats, Wistar
MH  - Sensory Receptor Cells/*metabolism
MH  - Sequence Analysis, RNA/methods
MH  - Transcriptome/*physiology
MH  - Trigeminal Ganglion/*metabolism
OTO - NOTNLM
OT  - RNA-Sequencing
OT  - cranial system
OT  - migraine
OT  - pain
OT  - sensory neurons
OT  - spinal system
EDAT- 2017/04/01 06:00
MHDA- 2018/02/07 06:00
CRDT- 2017/04/01 06:00
PHST- 2016/12/19 00:00 [received]
PHST- 2017/03/13 00:00 [revised]
PHST- 2017/03/16 00:00 [accepted]
PHST- 2017/04/01 06:00 [pubmed]
PHST- 2018/02/07 06:00 [medline]
PHST- 2017/04/01 06:00 [entrez]
AID - S0306-4522(17)30193-8 [pii]
AID - 10.1016/j.neuroscience.2017.03.027 [doi]
PST - ppublish
SO  - Neuroscience. 2017 May 14;350:169-179. doi: 10.1016/j.neuroscience.2017.03.027. 
      Epub 2017 Mar 27.