PMID- 28360392
OWN - NLM
STAT- MEDLINE
DCOM- 20170615
LR  - 20190923
IS  - 0973-7731 (Electronic)
IS  - 0022-1333 (Linking)
VI  - 96
IP  - 1
DP  - 2017 Mar
TI  - Transcript variations, phylogenetic tree and chromosomal localization of porcine 
      aryl hydrocarbon receptor (AhR) and AhR nuclear translocator (ARNT) genes.
PG  - 75-85
AB  - Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor best 
      known for mediating xenobiotic-induced toxicity. AhR requires aryl hydrocarbon 
      receptor nuclear translocator (ARNT) to form an active transcription complex and 
      promote the activation of genes which have dioxin responsive element in their 
      regulatory regions. The present study was performed to determine the complete 
      cDNA sequences of porcine AhR and ARNT genes and their chromosomal localization. 
      Total RNA from porcine livers were used to obtain the sequence of the entire 
      porcine transcriptome by next-generation sequencing (NGS; lllumina HiSeq2500). In 
      addition, both, in silico analysis and fluorescence in situ hybridization (FISH) 
      were used to determine chromosomal localization of porcine AhR and ARNT genes. In 
      silico analysis of nucleotide sequences showed that there were two transcript 
      variants of AhR and ARNT genes in the pig. In addition, computer analysis 
      revealed that AhR gene in the pig is located on chromosome 9 and ARNT on 
      chromosome 4. The results of FISH experiment confirmed the localization of 
      porcine AhR and ARNT genes. In the present study, for the first time, the full 
      cDNAs of AhR and ARNT were demonstrated in the pig. In future, it would be 
      interesting to determine the tissue distribution of AhR and ARNT transcript 
      variants in the pig and to test whether these variants are associated with 
      different biological functions and/or different activation pathways.
FAU - Sadowska, Agnieszka
AU  - Sadowska A
AD  - Department of Animal Physiology, University of Warmia and Mazury, Oczapowskiego 
      1A, 10-719 Olsztyn, Poland. agnieszka.sadowska@uwm.edu.pl.
FAU - Paukszto, Lukasz
AU  - Paukszto L
FAU - Nynca, Anna
AU  - Nynca A
FAU - Szczerbal, Izabela
AU  - Szczerbal I
FAU - Orlowska, Karina
AU  - Orlowska K
FAU - Swigonska, Sylwia
AU  - Swigonska S
FAU - Ruszkowska, Monika
AU  - Ruszkowska M
FAU - Molcan, Tomasz
AU  - Molcan T
FAU - Jastrzebski, Jan P
AU  - Jastrzebski JP
FAU - Panasiewicz, Grzegorz
AU  - Panasiewicz G
FAU - Ciereszko, Renata E
AU  - Ciereszko RE
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Genet
JT  - Journal of genetics
JID - 2985113R
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator/classification/*genetics
MH  - *Chromosome Mapping
MH  - Gene Frequency
MH  - *Genetic Variation
MH  - Open Reading Frames
MH  - *Phylogeny
MH  - RNA, Messenger/*genetics
MH  - Receptors, Aryl Hydrocarbon/classification/*genetics
MH  - Sequence Analysis, RNA
MH  - Swine
EDAT- 2017/04/01 06:00
MHDA- 2017/06/16 06:00
CRDT- 2017/04/01 06:00
PHST- 2017/04/01 06:00 [entrez]
PHST- 2017/04/01 06:00 [pubmed]
PHST- 2017/06/16 06:00 [medline]
AID - 10.1007/s12041-017-0745-3 [doi]
PST - ppublish
SO  - J Genet. 2017 Mar;96(1):75-85. doi: 10.1007/s12041-017-0745-3.