PMID- 28362944
OWN - NLM
STAT- MEDLINE
DCOM- 20171222
LR  - 20220316
IS  - 1537-6591 (Electronic)
IS  - 1058-4838 (Linking)
VI  - 64
IP  - 7
DP  - 2017 Apr 1
TI  - Zika Virus Infects Human Fetal Brain Microglia and Induces Inflammation.
PG  - 914-920
LID - 10.1093/cid/ciw878 [doi]
AB  - BACKGROUND: The unprecedented reemergence of Zika virus (ZIKV) has startled the 
      world with reports of increased microcephaly in Brazil. ZIKV can infect human 
      neural progenitors and impair brain growth. However, direct evidence of ZIKV 
      infection in human fetal brain tissues remains elusive. METHODS: Investigations 
      were performed with brain cell preparations obtained from 9 donors. Virus 
      infectivity was assessed by detection of virus antigen by flow cytometry together 
      with various hematopoietic cell surface markers. Virus replication was determined 
      by viral RNA quantification. Cytokine levels in supernatant obtained from 
      virus-infected fetal brain cells were measured simultaneously in microbead-based 
      immunoassays. RESULTS: We also show that ZIKV infection was particularly evident 
      in hematopoietic cells with microglia, the brain-resident macrophage population 
      being one of the main targets. Infection induces high levels of proinflammatory 
      immune mediators such as interleukin 6 (IL-6), tumor necrosis factor alpha 
      (TNF-alpha), interleukin 1beta (IL-1beta), and monocyte chemotactic protein 1 (MCP-1). 
      CONCLUSIONS: Our results highlight an important role for microglia and 
      neuroinflammation during congenital ZIKV pathogenesis.
CI  - (c) The Author 2017. Published by Oxford University Press for the Infectious 
      Diseases Society of America. All rights reserved. For permissions, e-mail: 
      journals.permissions@oup.com.
FAU - Lum, Fok-Moon
AU  - Lum FM
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Low, Donovan K S
AU  - Low DK
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Fan, Yiping
AU  - Fan Y
AD  - Department of Reproductive Medicine and.
FAU - Tan, Jeslin J L
AU  - Tan JJ
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Lee, Bernett
AU  - Lee B
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Chan, Jerry K Y
AU  - Chan JK
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
AD  - Department of Reproductive Medicine and.
AD  - KK Research Centre, KK Women's and Children's Hospital, and.
AD  - Cancer and Stem Cell Program, Duke-NUS Medical School, Singapore; and.
FAU - Renia, Laurent
AU  - Renia L
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Ginhoux, Florent
AU  - Ginhoux F
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
FAU - Ng, Lisa F P
AU  - Ng LF
AD  - Singapore Immunology Network, Agency for Science, Technology and Research 
      (A*STAR).
AD  - Institute of Infection and Global Health, University of Liverpool, United 
      Kingdom.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Infect Dis
JT  - Clinical infectious diseases : an official publication of the Infectious Diseases 
      Society of America
JID - 9203213
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Brain/pathology/virology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chlorocebus aethiops
MH  - Cytokines/metabolism
MH  - Encephalitis, Viral/immunology/metabolism/pathology/virology
MH  - Fetus
MH  - Humans
MH  - Macrophages/immunology/metabolism/virology
MH  - Microcephaly/etiology
MH  - Microglia/metabolism/*virology
MH  - Monocytes/immunology/metabolism/virology
MH  - Vero Cells
MH  - Viral Load
MH  - *Zika Virus
MH  - Zika Virus Infection/immunology/metabolism/pathology/*virology
OTO - NOTNLM
OT  - Zika virus
OT  - inflammation
OT  - microglia.
EDAT- 2017/04/01 06:00
MHDA- 2017/12/23 06:00
CRDT- 2017/04/01 06:00
PHST- 2016/10/12 00:00 [received]
PHST- 2017/01/04 00:00 [accepted]
PHST- 2017/04/01 06:00 [entrez]
PHST- 2017/04/01 06:00 [pubmed]
PHST- 2017/12/23 06:00 [medline]
AID - 3041121 [pii]
AID - 10.1093/cid/ciw878 [doi]
PST - ppublish
SO  - Clin Infect Dis. 2017 Apr 1;64(7):914-920. doi: 10.1093/cid/ciw878.