PMID- 28362976
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20181202
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Mar 16
TI  - Paracrine effect of bone marrow mesenchymal stem cells on proliferation, 
      apoptosis, and alpha-actin-2 expression in hepatic stellate cells.
LID - 10.4238/gmr16019201 [doi]
AB  - We investigated the paracrine effects of bone marrow mesenchymal stem cells 
      (BMSCs) on the proliferation, apoptosis, and alpha-actin-2 (ACTA2) expression of 
      hepatic stellate cells (HSCs), and explored the possible mechanisms of hepatocyte 
      growth factor (HGF). We established a co-culture system by culturing BMSCs on the 
      upper layer and HSCs on the lower layer of a 6-well Transwell plate. Normal HSCs 
      were cultured alone as a control. Cell apoptosis was determined by flow 
      cytometry. We detected the expression of ACTA2 mRNA and ACTA2 protein in HSC 
      using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and 
      western blotting, respectively. ACTA2 in HSCs was detected by fluorescent 
      staining, and HGF in the co-culture supernatant was detected by enzyme-linked 
      immunosorbent assay (ELISA). The apoptotic rate of HSCs in the experiment group 
      was 2.6 times that in the control group (P < 0.05). The expression levels of 
      ACTA2 mRNA and ACTA2 protein were significantly inhibited in HSCs compared with 
      the control group (P < 0.05). HGF concentration in the co-culture supernatant was 
      0.43 +/- 0.47 mM in the experimental group, which was significantly higher than in 
      the control group (0.16 +/- 0.43 mM) (P < 0.05). The paracrine effect of BMSCs, 
      which was caused by the suppression of ACTA2 and HGF expression, induced HSC 
      apoptosis.
FAU - Cao, H-J
AU  - Cao HJ
AD  - Department of Gastrointestinal Medicine, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Wang, M-D
AU  - Wang MD
AD  - Department of Emergency and Trauma Center, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, Hangzhou, China wangmudan_d@163.com.
FAU - Li, S-G
AU  - Li SG
AD  - Department of Gastrointestinal Medicine, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Zhu, L
AU  - Zhu L
AD  - Department of Gastrointestinal Medicine, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Zheng, J-H
AU  - Zheng JH
AD  - Department of Gastrointestinal Medicine, The First Affiliated Hospital of 
      Zhejiang Chinese Medical University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20170316
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (ACTA2 protein, human)
RN  - 0 (Actins)
RN  - 0 (HGF protein, human)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Actins/*genetics/*metabolism
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Down-Regulation
MH  - Hepatic Stellate Cells/*cytology/metabolism
MH  - Hepatocyte Growth Factor/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Paracrine Communication
EDAT- 2017/04/01 06:00
MHDA- 2017/04/07 06:00
CRDT- 2017/04/01 06:00
PHST- 2017/04/01 06:00 [entrez]
PHST- 2017/04/01 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - gmr-16-01-gmr.16019201 [pii]
AID - 10.4238/gmr16019201 [doi]
PST - epublish
SO  - Genet Mol Res. 2017 Mar 16;16(1). doi: 10.4238/gmr16019201.