PMID- 28363014 OWN - NLM STAT- MEDLINE DCOM- 20170804 LR - 20181107 IS - 1520-5851 (Electronic) IS - 0013-936X (Linking) VI - 51 IP - 8 DP - 2017 Apr 18 TI - Chromosomal Aberrations in Large Japanese Field Mice (Apodemus speciosus) Captured near Fukushima Dai-ichi Nuclear Power Plant. PG - 4632-4641 LID - 10.1021/acs.est.6b06210 [doi] AB - Since the Fukushima Dai-ichi Nuclear Power Plant accident, radiation effects on nonhuman biota in the contaminated areas have been a major concern. Here, we analyzed the frequencies of chromosomal aberrations (translocations and dicentrics) in the splenic lymphocytes of large Japanese field mice (Apodemus speciosus) inhabiting Fukushima Prefecture. A. speciosus chromosomes 1, 2, and 5 were flow-sorted in order to develop A. speciosus chromosome-specific painting probes, and FISH (fluorescence in situ hybridization) was performed using these painting probes to detect the translocations and dicentrics. The average frequency of the translocations and dicentrics per cell in the heavily contaminated area was significantly higher than the frequencies in the case of the noncontaminated control area and the slightly and moderately contaminated areas, and this aberration frequency in individual mice tended to roughly increase with the estimated dose rates and accumulated doses. In all four sampling areas, the proportion of aberrations occurring in chromosome 2 was approximately >3 times higher than that in chromosomes 1 and 5, which suggests that A. speciosus chromosome 2 harbors a fragile site that is highly sensitive to chromosome breaks induced by cellular stress such as DNA replication. The elevated frequency of chromosomal aberrations in A. speciosus potentially resulting from the presence of a fragile site in chromosome 2 might make it challenging to observe the mild effect of chronic low-dose-rate irradiation on the induction of chromosomal aberrations in A. speciosus inhabiting the contaminated areas of Fukushima. FAU - Kawagoshi, Taiki AU - Kawagoshi T AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Shiomi, Naoko AU - Shiomi N AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Takahashi, Hiroyuki AU - Takahashi H AD - Tokyo Nuclear Services Co., Ltd. 1-3-5 Taito, Taito-ku, Tokyo 110-0016, Japan. FAU - Watanabe, Yoshito AU - Watanabe Y AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Fuma, Shoichi AU - Fuma S AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Doi, Kazutaka AU - Doi K AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Kawaguchi, Isao AU - Kawaguchi I AD - Center for Radiation Protection Knowledge, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Aoki, Masanari AU - Aoki M AD - Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan. FAU - Kubota, Masahide AU - Kubota M AD - Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan. FAU - Furuhata, Yoshiaki AU - Furuhata Y AD - Japan Wildlife Research Center, 3-3-7 Koutoubashi, Sumida-ku, Tokyo 130-8606, Japan. FAU - Shigemura, Yusaku AU - Shigemura Y AD - Japan NUS Co., Ltd, 7-5-25 Nishi-shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. FAU - Mizoguchi, Masahiko AU - Mizoguchi M AD - Japan NUS Co., Ltd, 7-5-25 Nishi-shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. FAU - Yamada, Fumio AU - Yamada F AD - Forestry and Forest Products Research Institute , 1 Matsunosato, Tsukuba, Ibaraki 305-8687, Japan. FAU - Tomozawa, Morihiko AU - Tomozawa M AD - Department of Biology, Keio University , 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8521, Japan. FAU - Sakamoto, Shinsuke H AU - Sakamoto SH AD - Faculty of Agriculture, University of Miyazaki , Kibana Campus, Miyazaki 889-2192, Japan. FAU - Yoshida, Satoshi AU - Yoshida S AD - Department of Management and Planning, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. FAU - Kubota, Yoshihisa AU - Kubota Y AUID- ORCID: 0000-0002-2807-1535 AD - Fukushima Project Headquarters, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Science and Technology , 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20170407 PL - United States TA - Environ Sci Technol JT - Environmental science & technology JID - 0213155 SB - IM CIN - Environ Sci Technol. 2017 Jul 18;51(14):8198-8199. PMID: 28654247 CIN - Environ Sci Technol. 2017 Jul 18;51(14):8196-8197. PMID: 28654249 MH - Animals MH - *Chromosome Aberrations MH - *Fukushima Nuclear Accident MH - In Situ Hybridization, Fluorescence MH - Mice MH - Murinae/*genetics MH - *Nuclear Power Plants EDAT- 2017/04/01 06:00 MHDA- 2017/08/05 06:00 CRDT- 2017/04/01 06:00 PHST- 2017/04/01 06:00 [pubmed] PHST- 2017/08/05 06:00 [medline] PHST- 2017/04/01 06:00 [entrez] AID - 10.1021/acs.est.6b06210 [doi] PST - ppublish SO - Environ Sci Technol. 2017 Apr 18;51(8):4632-4641. doi: 10.1021/acs.est.6b06210. Epub 2017 Apr 7.