PMID- 28363251 OWN - NLM STAT- MEDLINE DCOM- 20170713 LR - 20191210 IS - 1943-4936 (Electronic) IS - 1040-6387 (Linking) VI - 29 IP - 3 DP - 2017 May TI - Assessment of air sampling methods and size distribution of virus-laden aerosols in outbreaks in swine and poultry farms. PG - 298-304 LID - 10.1177/1040638717700221 [doi] AB - Swine and poultry viruses, such as porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and highly pathogenic avian influenza virus (HPAIV), are economically important pathogens that can spread via aerosols. The reliability of methods for quantifying particle-associated viruses as well as the size distribution of aerosolized particles bearing these viruses under field conditions are not well documented. We compared the performance of 2 size-differentiating air samplers in disease outbreaks that occurred in swine and poultry facilities. Both air samplers allowed quantification of particles by size, and measured concentrations of PRRSV, PEDV, and HPAIV stratified by particle size both within and outside swine and poultry facilities. All 3 viruses were detectable in association with aerosolized particles. Proportions of positive sampling events were 69% for PEDV, 61% for HPAIV, and 8% for PRRSV. The highest virus concentrations were found with PEDV, followed by HPAIV and PRRSV. Both air collectors performed equally for the detection of total virus concentration. For all 3 viruses, higher numbers of RNA copies were associated with larger particles; however, a bimodal distribution of particles was observed in the case of PEDV and HPAIV. FAU - Alonso, Carmen AU - Alonso C AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Raynor, Peter C AU - Raynor PC AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Goyal, Sagar AU - Goyal S AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Olson, Bernard A AU - Olson BA AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Alba, Anna AU - Alba A AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Davies, Peter R AU - Davies PR AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. FAU - Torremorell, Montserrat AU - Torremorell M AD - Department of Veterinary Population Medicine, College of Veterinary Medicine (Alonso, Alba, Davies, Torremorell), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Division of Environmental Health Sciences, School of Public Health (Raynor), University of Minnesota-Twin Cities, Minneapolis, MN. AD - Department of Mechanical Engineering, College of Science and Engineering (Olson), University of Minnesota-Twin Cities, Minneapolis, MN. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20170331 PL - United States TA - J Vet Diagn Invest JT - Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc JID - 9011490 RN - 0 (Aerosols) SB - IM MH - Aerosols MH - *Air Microbiology MH - *Animal Husbandry MH - Animals MH - Disease Outbreaks/*veterinary MH - Farms MH - Influenza A virus/isolation & purification MH - Porcine epidemic diarrhea virus/isolation & purification MH - Porcine respiratory and reproductive syndrome virus/isolation & purification MH - Poultry MH - Reproducibility of Results MH - Swine MH - Swine Diseases/*transmission/virology OTO - NOTNLM OT - Aerosols OT - air sampler OT - animal viruses OT - cascade impactor OT - disease transmission OT - particle size EDAT- 2017/04/02 06:00 MHDA- 2017/07/14 06:00 CRDT- 2017/04/02 06:00 PHST- 2017/04/02 06:00 [pubmed] PHST- 2017/07/14 06:00 [medline] PHST- 2017/04/02 06:00 [entrez] AID - 10.1177/1040638717700221 [doi] PST - ppublish SO - J Vet Diagn Invest. 2017 May;29(3):298-304. doi: 10.1177/1040638717700221. Epub 2017 Mar 31.