PMID- 28363573
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20181202
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 165
DP  - 2017 Jun 1
TI  - Effects of fucoidans and heparin on reactions of neutrophils induced by IL-8 and 
      C5a.
PG  - 462-469
LID - S0144-8617(17)30181-9 [pii]
LID - 10.1016/j.carbpol.2017.02.051 [doi]
AB  - Fucose-containing sulfated polysaccharides (fucoidans) from brown algae exhibit 
      anti-inflammatory activity in vivo, however, there is only limited knowledge 
      about their mode of action. Potential targets may be the chemokine interleukin 8 
      (IL-8) and the anaphylatoxin C5a, as they are closely linked to inflammatory 
      processes. In this study, two fucoidans from Saccharina latissima and Fucus 
      vesiculosus, and unfractionated heparin (UFH) were examined for their binding 
      properties to IL-8 and C5a and their effects on IL-8- and C5a-induced reactions 
      of polymorphonuclear neutrophils (PMN). As proved by a competitive sulfated 
      polysaccharide-coating-ELISA, both fucoidans bind to IL-8 and C5a, whereby they 
      showed higher affinity to IL-8. Whereas UFH displayed only moderate effects, the 
      fucoidans concentration-dependently reduced the IL-8- and C5a-induced 
      intracellular calcium release, Erk1/2 phosphorylation and chemotaxis of PMN. 
      Their inhibitory potency is dependent on the target protein, but also other 
      aspects than the binding turned out to play a role.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Liewert, Inga
AU  - Liewert I
AD  - Pharmaceutical Institute, Kiel University, Gutenbergstrasse 76, 24118 Kiel, 
      Germany. Electronic address: iliewert@pharmazie.uni-kiel.de.
FAU - Ehrig, Karina
AU  - Ehrig K
AD  - Pharmaceutical Institute, Kiel University, Gutenbergstrasse 76, 24118 Kiel, 
      Germany. Electronic address: kehrig@pharmazie.uni-kiel.de.
FAU - Alban, Susanne
AU  - Alban S
AD  - Pharmaceutical Institute, Kiel University, Gutenbergstrasse 76, 24118 Kiel, 
      Germany. Electronic address: salban@pharmazie.uni-kiel.de.
LA  - eng
PT  - Journal Article
DEP - 20170220
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Interleukin-8)
RN  - 0 (Polysaccharides)
RN  - 80295-54-1 (Complement C5a)
RN  - 9005-49-6 (Heparin)
RN  - 9072-19-9 (fucoidan)
SB  - IM
MH  - Complement C5a
MH  - Heparin/*pharmacology
MH  - Humans
MH  - Interleukin-8
MH  - Neutrophils/*drug effects
MH  - Polysaccharides/*pharmacology
OTO - NOTNLM
OT  - C5a
OT  - Fucoidan
OT  - Fucoidan (PubChem CID: 92023653)
OT  - Heparin
OT  - Interleukin 8
OT  - Sodium Heparin (PubChem CID: 22833565)
EDAT- 2017/04/02 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/04/02 06:00
PHST- 2016/12/23 00:00 [received]
PHST- 2017/02/02 00:00 [revised]
PHST- 2017/02/14 00:00 [accepted]
PHST- 2017/04/02 06:00 [entrez]
PHST- 2017/04/02 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
AID - S0144-8617(17)30181-9 [pii]
AID - 10.1016/j.carbpol.2017.02.051 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2017 Jun 1;165:462-469. doi: 10.1016/j.carbpol.2017.02.051. Epub 
      2017 Feb 20.