PMID- 28364599
OWN - NLM
STAT- MEDLINE
DCOM- 20180220
LR  - 20191210
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 90
DP  - 2017 Jun
TI  - Flavonoids from Enicostema littorale blume enhances glucose uptake of cells in 
      insulin resistant human liver cancer (HepG2) cell line via IRS-1/PI3K/Akt 
      pathway.
PG  - 268-277
LID - S0753-3322(16)32973-0 [pii]
LID - 10.1016/j.biopha.2017.03.047 [doi]
AB  - Diabetes mellitus has spread over the world with 347 million people affected. 
      Insulin resistance is a main pathogenic event in Type 2 Diabetes Mellitus (T2DM) 
      leading to a reduction in glucose uptake by peripheral tissue and increased 
      hepatic glucose output. In this study, we have isolated four flavonoid rich 
      fractions fraction A (FA), fraction B (FB), fraction C (FC) and fraction D (FD) 
      from Enicostema littorale. All the fractions were preliminary screened for TLC 
      fingerprinting, total flavonoid content. Total eight flavonoids were identified 
      by LC/MS. Insulin resistant HepG2 (IR/HepG2) model was established by inducing 
      insulin resistance in HepG2 cells to investigate the effect of these fractions on 
      IR/HepG2 cell line for their glucose uptake. The results showed the significant 
      dose dependant increase in glucose uptake of cells treated with FD. It showed 
      significant activity at a concentration of 10mug/ml. The LC/MS results of FD 
      demonstrated the presence of C-glycoside Swertisin which could be responsible for 
      the effect. Further, to investigate the mechanism of action, gene expression for 
      insulin receptor substrate 1 (IRS-1), protein kinase B (Akt-2) and glucose 
      transporter 4 (GLUT-4) genes were evaluated by real time PCR. A significant 
      upregulation of these genes was observed in FD treated samples, thereby 
      indicating the enhancement of glucose uptake rate of cells via IRS-1/PI3K/Akt 
      pathway.
CI  - Copyright (c) 2017 Elsevier Masson SAS. All rights reserved.
FAU - Mokashi, Priyanka
AU  - Mokashi P
AD  - Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS 
      University, Vile Parle (West), Mumbai, 400056, India.
FAU - Khanna, Aparna
AU  - Khanna A
AD  - Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS 
      University, Vile Parle (West), Mumbai, 400056, India.
FAU - Pandita, Nancy
AU  - Pandita N
AD  - Department of Chemistry, Sunandan Divatia School of Science, NMIMS University, 
      Vile Parle (West), Mumbai, 400056, India. Electronic address: 
      nancy.pandita@nmims.edu.
LA  - eng
PT  - Journal Article
DEP - 20170330
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (C-glycoside)
RN  - 0 (Flavonoids)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Glycosides)
RN  - 0 (IRS1 protein, human)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Monosaccharides)
RN  - 0 (Plant Extracts)
RN  - EC 2.7.10.1 (Receptor, Insulin)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cell Line, Tumor
MH  - Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Flavonoids/*pharmacology
MH  - Gentianaceae/*chemistry
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4/metabolism
MH  - Glycosides
MH  - Hep G2 Cells
MH  - Humans
MH  - Insulin/metabolism
MH  - Insulin Receptor Substrate Proteins/*metabolism
MH  - Insulin Resistance/*physiology
MH  - Liver Neoplasms
MH  - Monosaccharides/pharmacology
MH  - Phosphatidylinositol 3-Kinases/*metabolism
MH  - Phosphorylation/drug effects
MH  - Plant Extracts/pharmacology
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Receptor, Insulin/metabolism
MH  - Signal Transduction/drug effects
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - E. littorale
OT  - Flavonoids
OT  - Glucose transporters
OT  - Glucose uptake assay
OT  - HepG2 cell line
OT  - Insulin receptors
OT  - LC/MS
EDAT- 2017/04/02 06:00
MHDA- 2018/02/21 06:00
CRDT- 2017/04/02 06:00
PHST- 2016/12/29 00:00 [received]
PHST- 2017/03/03 00:00 [revised]
PHST- 2017/03/18 00:00 [accepted]
PHST- 2017/04/02 06:00 [pubmed]
PHST- 2018/02/21 06:00 [medline]
PHST- 2017/04/02 06:00 [entrez]
AID - S0753-3322(16)32973-0 [pii]
AID - 10.1016/j.biopha.2017.03.047 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2017 Jun;90:268-277. doi: 10.1016/j.biopha.2017.03.047. Epub 
      2017 Mar 30.