PMID- 28364790
OWN - NLM
STAT- MEDLINE
DCOM- 20170426
LR  - 20220330
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 63
IP  - 2
DP  - 2017 Feb 28
TI  - The effects of melatonin on oxidative stress and prevention of primordial 
      follicle loss via activation of mTOR pathway in the rat ovary.
PG  - 100-106
LID - 10.14715/cmb/2017.63.2.16 [doi]
AB  - Mammalian Target of Rapamycin (mTOR) signaling pathway has important roles in the 
      regulation of puberty onset, gonadotropin secretion, follicular development and 
      ovulation. Melatonin (N-acetyl-5-methoxytryptamine) is a lipophilic hormone has 
      multiple functions in regulating the fertility. Recent studies have shown that 
      melatonin affected the number or maturation of follicles in the ovary. The aim of 
      this study was to investigate the effects of melatonin on mTOR expression and 
      quantity of follicle in rat ovary. In the present study, a total of 45 female 
      rats were randomly divided into three groups. Group 1; Control (C), Group 2: 
      Vehicle (V) and Group 3; Melatonin (M). Melatonin was administered 
      intraperitoneally at a dose of 50 mg/kg/day for 30 days in Melatonin group. The 
      effects of Melatonin on the expression of mTOR and downstream components  were 
      determined by Western Blot and Reverse Transcriptase PCR analysis. Upon Western 
      Blot and RT-PCR evaluations, we detected higher expression and activation of 
      mTOR, P70S6K, PKCalpha, PCNA and higher numbers of primordial  follicles in 
      melatonin group compared with V and C group. In addition to this results, 
      melatonin decreased oxidative stress markers, such as MDA, on the contrary, 
      levels of antioxidative markers, such as CAT and GPx, were increased by melatonin 
      in rat ovary. This study indicated that melatonin may have a significant 
      protective effect on primordial follicles and increase the expression of mTOR and 
      downstream components in rat ovary. Melatonin treatment may have a beneficial 
      effect on fertility.
FAU - Behram Kandemir, Y
AU  - Behram Kandemir Y
AD  - Department of Anatomy, Akdeniz University Faculty of Medicine, Antalya, Turkey.
FAU - Aydin, C
AU  - Aydin C
AD  - Department of Nursing, Bucak School of Health, Mehmet Akif Ersoy University, 
      Burdur, Turkey.
FAU - Gorgisen, G
AU  - Gorgisen G
AD  - Department of Medical Biology, Yuzuncu Yil University Faculty of Medicine, Van, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20170228
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Antioxidants)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C-alpha)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/administration & dosage/pharmacology
MH  - Blotting, Western
MH  - Catalase/metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Glutathione Peroxidase/metabolism
MH  - Injections, Intraperitoneal
MH  - Malondialdehyde/metabolism
MH  - Melatonin/administration & dosage/*pharmacology
MH  - Ovarian Follicle/*drug effects/metabolism
MH  - Ovary/*drug effects/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - Protein Kinase C-alpha/genetics/metabolism
MH  - Rats, Wistar
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribosomal Protein S6 Kinases, 70-kDa/genetics/metabolism
MH  - Signal Transduction/*drug effects/genetics
MH  - Superoxide Dismutase/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - Follicle
OT  - Infertility.
OT  - Melatonin
OT  - Ovary
OT  - mTOR
EDAT- 2017/04/04 06:00
MHDA- 2017/04/27 06:00
CRDT- 2017/04/03 06:00
PHST- 2017/03/26 00:00 [received]
PHST- 2017/04/03 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/04/27 06:00 [medline]
AID - 10.14715/cmb/2017.63.2.16 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2017 Feb 28;63(2):100-106. doi: 
      10.14715/cmb/2017.63.2.16.