PMID- 28365681
OWN - NLM
STAT- MEDLINE
DCOM- 20170623
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 41
IP  - 5
DP  - 2017
TI  - Bone Marrow-Derived Mononuclear Cell Therapy Accelerates Renal 
      Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and 
      Apoptotic Related Molecules.
PG  - 1736-1752
LID - 10.1159/000471866 [doi]
AB  - BACKGROUND/AIMS: We investigated the regenerative capacity of intravenous 
      administration of bone marrow-derived mononuclear cells (BMMCs) in a rat model of 
      bilateral renal ischemia/reperfusion (IR) injury and the involvement of 
      inflammatory anti-inflammatory and other biological markers in this process. 
      METHODS: Rats were subjected to 1h bilateral renal pedicle clamping. BMMCs were 
      injected i.v 1h after reperfusion and tracked by 99mTc and GFP+ BMMCs. 
      Twenty-four hours after reperfusion, renal function and histological changes were 
      evaluated. The mRNA (real time PCR) and protein (ELISA and immuno-staining) 
      expression of biological markers were analyzed. RESULTS: Renal function and 
      structure improved after infusion of BMMCs in the IR group (IR-C). Labeled BMMCs 
      were found in the kidneys after therapy. The expression of inflammatory and 
      biological markers (TLR-2, TRL-4, RAGE, IL-17, HMGB-1, KIM-1) were reduced and 
      the expression of anti-inflammatory and antioxidant markers (IL-10, Nrf2, and 
      HO-1) were increased in IR-C animals compared with IR untreated animals (IR-S). 
      The apoptotic index diminished and the proliferation index increased in IR-C 
      compared with IR-S. CONCLUSION: The results contribute to our understanding of 
      the role of different biological players in morphofunctional renal improvement 
      and cytoprotection in a post-ischemic reperfusion kidney injury model subjected 
      to cellular therapy.
CI  - (c) 2017 The Author(s)Published by S. Karger AG, Basel.
FAU - Ornellas, Felipe Mateus
AU  - Ornellas FM
AD  - Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
AD  - Laboratory of Immunopathology, Rio de Janeiro, Brazil.
FAU - Ornellas, Debora Santos
AU  - Ornellas DS
AD  - Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
FAU - Martini, Sabrina Vargas
AU  - Martini SV
AD  - Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
FAU - Castiglione, Raquel Carvalho
AU  - Castiglione RC
AD  - Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
AD  - Laboratory for Clinical and Experimental Research on Vascular Biology, Biomedical 
      Center, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
FAU - Ventura, Grasiella Maria
AU  - Ventura GM
AD  - Confocal Microscopy Unit, Institute of Biomedical Science, Rio de Janeiro, 
      Brazil.
FAU - Rocco, Patricia R
AU  - Rocco PR
AD  - Laboratory of Pulmonary Investigation, Institute of Biophysics Carlos Chagas 
      Filho, Rio de Janeiro, Brazil.
FAU - Gutfilen, Bianca
AU  - Gutfilen B
AD  - Department of Radiology, School of Medicine, Federal University of Rio de 
      Janeiro, Rio de Janeiro, Brazil.
FAU - de Souza, Sergio A
AU  - de Souza SA
AD  - Department of Radiology, School of Medicine, Federal University of Rio de 
      Janeiro, Rio de Janeiro, Brazil.
FAU - Takiya, Christina Maeda
AU  - Takiya CM
AD  - Laboratory of Immunopathology, Rio de Janeiro, Brazil.
FAU - Morales, Marcelo Marcos
AU  - Morales MM
AD  - Laboratory of Cellular and Molecular Physiology, Rio de Janeiro, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170331
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Antioxidants)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Biomarkers)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Allografts
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/metabolism/pathology
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Inflammation/metabolism/pathology/therapy
MH  - Inflammation Mediators/*metabolism
MH  - *Kidney Diseases/metabolism/pathology/therapy
MH  - Rats
MH  - Rats, Wistar
MH  - *Reperfusion Injury/metabolism/pathology/therapy
OTO - NOTNLM
OT  - Bone marrow
OT  - Cellular therapy
OT  - Ischemia- reperfusion
OT  - Renal
EDAT- 2017/04/04 06:00
MHDA- 2017/06/24 06:00
CRDT- 2017/04/03 06:00
PHST- 2016/11/07 00:00 [received]
PHST- 2017/03/08 00:00 [accepted]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/06/24 06:00 [medline]
PHST- 2017/04/03 06:00 [entrez]
AID - 000471866 [pii]
AID - 10.1159/000471866 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2017;41(5):1736-1752. doi: 10.1159/000471866. Epub 2017 Mar 
      31.