PMID- 28365872 OWN - NLM STAT- MEDLINE DCOM- 20180312 LR - 20181113 IS - 1573-2576 (Electronic) IS - 0360-3997 (Print) IS - 0360-3997 (Linking) VI - 40 IP - 3 DP - 2017 Jun TI - Saturated Fatty Acid Increases Lung Macrophages and Augments House Dust Mite-Induced Airway Inflammation in Mice Fed with High-Fat Diet. PG - 1072-1086 LID - 10.1007/s10753-017-0550-4 [doi] AB - Obesity is one of the phenotypes of severe asthma, which is considered to be a heterogeneous syndrome; however, its interaction with airway inflammation is not fully understood. The aim of this study was to clarify the role of saturated fatty acids in augmenting airway inflammation induced by house dust mite (HDM) in obesity. Subjects were Balb/c mice fed a high-fat diet (HFD) for 10 weeks, followed by sensitization and exposure to HDM. Subjects were also administered palmitic acid (PA) for 4 weeks with concurrent sensitization and exposure to HDM. Airway inflammation was assessed by quantifying the amount of inflammatory cells in bronchoalveolar lavage (BAL) and airway resistance was measured. In vitro, lipopolysaccharide (LPS)-primed macrophages were stimulated by PA. The amount of monocyte chemoattractant protein-1 (MCP-1), interleukin-1beta (IL-1beta), and tumor necrosis factor alpha (TNF-alpha) was examined in the supernatant. Compared to normal chow mice, HFD mice underwent significant increases in body weight; increases in number of lung macrophages, including circulating monocytes and alveolar macrophages; and increases in bronchoalveolar lavage fluid (BALF) total cell count, including neutrophils but not eosinophils, after HDM sensitization and exposure. In vitro, PA induced MCP-1 and augmented LPS-primed production of IL-1beta and TNF-alpha in macrophages. Among HDM mice that were administered PA, there was an increase BALF total cell count, including neutrophils but not eosinophils, compared to vehicle mice. In conclusion, saturated fatty acid increased the number of lung macrophages and augmented HDM-induced neutrophilic airway inflammation in a HFD mouse model. FAU - Tashiro, Hiroki AU - Tashiro H AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. FAU - Takahashi, Koichiro AU - Takahashi K AUID- ORCID: 0000-0003-0461-5072 AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. takahak@cc.saga-u.ac.jp. FAU - Sadamatsu, Hironori AU - Sadamatsu H AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. FAU - Kato, Go AU - Kato G AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. FAU - Kurata, Keigo AU - Kurata K AD - Institute of Tokyo Environmental Allergy, Tokyo, Japan. FAU - Kimura, Shinya AU - Kimura S AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. FAU - Sueoka-Aragane, Naoko AU - Sueoka-Aragane N AD - Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga, 849-8501, Japan. LA - eng PT - Journal Article PL - United States TA - Inflammation JT - Inflammation JID - 7600105 RN - 0 (Cytokines) RN - 0 (Fatty Acids) SB - IM MH - Animals MH - Asthma/*pathology MH - Cell Count MH - Cytokines/drug effects MH - Diet, High-Fat MH - Eosinophils MH - Fatty Acids/*pharmacology MH - Inflammation/*chemically induced MH - Macrophages, Alveolar/cytology/*drug effects MH - Mice MH - Neutrophils MH - Pyroglyphidae PMC - PMC5429367 OTO - NOTNLM OT - bronchial asthma OT - high-fat diet OT - house dust mite OT - macrophages OT - obesity OT - saturated fatty acid COIS- FUNDING: This study was supported by the Researcher Supporting Program of Saga University (KT). CONFLICT OF INTEREST: The authors declare that they have no competing interests. ETHICAL APPROVAL: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. EDAT- 2017/04/04 06:00 MHDA- 2018/03/13 06:00 PMCR- 2017/04/01 CRDT- 2017/04/03 06:00 PHST- 2017/04/04 06:00 [pubmed] PHST- 2018/03/13 06:00 [medline] PHST- 2017/04/03 06:00 [entrez] PHST- 2017/04/01 00:00 [pmc-release] AID - 10.1007/s10753-017-0550-4 [pii] AID - 550 [pii] AID - 10.1007/s10753-017-0550-4 [doi] PST - ppublish SO - Inflammation. 2017 Jun;40(3):1072-1086. doi: 10.1007/s10753-017-0550-4.