PMID- 28366194
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20181202
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 19
IP  - 5
DP  - 2017 May
TI  - Effects of a defined xeno-free medium on the growth and neurotrophic and 
      angiogenic properties of human adult stem cells.
PG  - 629-639
LID - S1465-3249(17)30424-3 [pii]
LID - 10.1016/j.jcyt.2017.02.360 [doi]
AB  - BACKGROUND: The growth properties and neurotrophic and angiogenic effects of 
      human mesenchymal stromal cells (MSCs) cultured in a defined xeno-free, 
      serum-free medium (MesenCult-XF) were investigated. METHODS: Human MSCs from 
      adipose tissue (ASCs) and bone marrow (BMSCs) were cultured in Minimum Essential 
      Medium-alpha (alpha-MEM) containing fetal calf serum or in MesenCult-XF. 
      Proliferation was measured over 10 passages and the colony-forming unit (CFU) 
      assay and expression of cluster of differentiation (CD) surface markers were 
      determined. Neurite outgrowth and angiogenic activity of the MSCs were 
      determined. RESULTS: At early passage, both ASCs and BMSCs showed better 
      proliferation in MesenCult-XF compared with standard alpha-MEM-containing serum. 
      However, CFUs were significantly lower in MesenCult-XF. ASCs cultured in 
      MesenCult-XF continued to expand at faster rates than cells grown in serum. BMSCs 
      showed morphological changes at late passage in MesenCult-XF and stained positive 
      for senescence beta-galactosidase activity. Expression levels of CD73 and CD90 were 
      similar in both cell types under the various culture conditions but CD105 was 
      significantly reduced at passage 10 in MesenCult-XF. In vitro stimulation of the 
      cells enhanced the expression of brain-derived neurotrophic factor (BDNF), 
      vascular endothelial growth factor (VEGF-A) and angiopoietin-1. Stimulated ASCs 
      grown in MesenCult-XF evoked the longest neurite outgrowth in a neuron co-culture 
      model. Stimulated BMSCs grown in MesenCult-XF produced the most extensive network 
      of capillary-like tube structures in an in vitro angiogenesis assay. CONCLUSIONS: 
      ASCs and BMSCs exhibit high levels of neurotrophic and angiogenic activity when 
      grown in the defined serum-free medium indicating their suitability for treatment 
      of various neurological conditions. However, long-term expansion in MesenCult-XF 
      might be restricted to ASCs.
CI  - Copyright (c) 2017 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Brohlin, Maria
AU  - Brohlin M
AD  - Division of Clinical Immunology and Transfusion Medicine, Tissue Establishment, 
      Cell Therapy Unit, Department of Laboratory Medicine, Umea University Hospital, 
      Umea, Sweden. Electronic address: maria.brohlin@vll.se.
FAU - Kelk, Peyman
AU  - Kelk P
AD  - Department of Integrative Medical Biology, Anatomy, Umea University, Umea, 
      Sweden.
FAU - Wiberg, Mikael
AU  - Wiberg M
AD  - Department of Integrative Medical Biology, Anatomy, Umea University, Umea, 
      Sweden; Department of Surgical and Perioperative Sciences, Hand and Plastic 
      Surgery, Umea University, Umea, Sweden.
FAU - Kingham, Paul J
AU  - Kingham PJ
AD  - Department of Integrative Medical Biology, Anatomy, Umea University, Umea, 
      Sweden.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Culture Media)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adipogenesis/drug effects
MH  - Adipose Tissue/cytology
MH  - Adult
MH  - Adult Stem Cells/*cytology/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Culture Media
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology
MH  - Neovascularization, Physiologic/*drug effects
MH  - Nerve Growth Factors/*pharmacology
MH  - Neurites/drug effects/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - adipose
OT  - bone marrow
OT  - clinical cell culture
OT  - mesenchymal stromal cells
OT  - neurotrophic factors
EDAT- 2017/04/04 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/04/04 06:00
PHST- 2016/09/20 00:00 [received]
PHST- 2017/02/22 00:00 [revised]
PHST- 2017/02/24 00:00 [accepted]
PHST- 2017/04/04 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
AID - S1465-3249(17)30424-3 [pii]
AID - 10.1016/j.jcyt.2017.02.360 [doi]
PST - ppublish
SO  - Cytotherapy. 2017 May;19(5):629-639. doi: 10.1016/j.jcyt.2017.02.360.