PMID- 28366212
OWN - NLM
STAT- MEDLINE
DCOM- 20180615
LR  - 20181202
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 965
DP  - 2017 May 1
TI  - A new configuration for bar adsorptive microextraction (BAmuE) for the 
      quantification of biomarkers (hexanal and heptanal) in human urine by HPLC 
      providing an alternative for early lung cancer diagnosis.
PG  - 54-62
LID - S0003-2670(17)30251-9 [pii]
LID - 10.1016/j.aca.2017.02.034 [doi]
AB  - In this paper, a remodeling of the bar adsorptive microextraction (BAmuE) 
      technique is proposed with impregnation of the derivatization reagent on the 
      surface of the adsorptive bar containing a biosorbent material. The 
      derivatization reagent was 2,4-dinitrophenylhydrazine (DNPH), which was adsorbed 
      on the surface of the bar containing cork powder as the extractor phase for the 
      determination of two aldehydes (hexanal and heptanal) which are known as lung 
      cancer biomarkers in human urine samples. The derivatization reaction and the 
      extraction occurred simultaneously on the surface of the bar (length 7.5 mm) 
      under acidic conditions. The method optimization was carried out by univariate 
      and multivariate analysis. The optimal conditions for the method were a DNPH to 
      aldehydes ratio of 40:1, buffer solution of pH 4.0, extraction time of 60 min and 
      liquid desorption of 10 min in 100 muL of acetonitrile. The aldehydes were 
      analyzed by HPLC-DAD with a simple and fast (6 min) chromatographic run. The 
      limits of detection (LODs) for hexanal and heptanal were 1.00 and 
      0.73 mumol L(-1), respectively. The relative recoveries in urine samples ranged 
      from 88 to 111% with relative standard deviations (RSDs) being less than 7%. The 
      method developed is of low cost and can be successfully used for the 
      quantification of these two lung cancer biomarkers in human urine samples, 
      potentially providing an early diagnosis of lung cancer.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Oenning, Anderson Luiz
AU  - Oenning AL
AD  - Departamento de Quimica, Universidade Federal de Santa Catarina, Florianopolis 
      88040900, SC, Brazil.
FAU - Mores, Lucas
AU  - Mores L
AD  - Departamento de Quimica, Universidade Federal de Santa Catarina, Florianopolis 
      88040900, SC, Brazil.
FAU - Dias, Adriana Neves
AU  - Dias AN
AD  - Departamento de Quimica, Universidade Federal de Santa Catarina, Florianopolis 
      88040900, SC, Brazil.
FAU - Carasek, Eduardo
AU  - Carasek E
AD  - Departamento de Quimica, Universidade Federal de Santa Catarina, Florianopolis 
      88040900, SC, Brazil. Electronic address: eduardo.carasek@ufsc.br.
LA  - eng
PT  - Journal Article
DEP - 20170306
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Aldehydes)
RN  - 0 (Biomarkers, Tumor)
RN  - 92N104S3HF (heptanal)
RN  - 9DC2K31JJQ (n-hexanal)
SB  - IM
MH  - Aldehydes/*urine
MH  - Biomarkers, Tumor/urine
MH  - Chromatography, High Pressure Liquid
MH  - *Early Detection of Cancer
MH  - Humans
MH  - *Liquid Phase Microextraction
MH  - Lung Neoplasms/*diagnosis
OTO - NOTNLM
OT  - Bar adsorptive microextraction
OT  - Cork
OT  - Heptanal
OT  - Hexanal
OT  - Lung cancer
OT  - Urine
EDAT- 2017/04/04 06:00
MHDA- 2018/06/16 06:00
CRDT- 2017/04/04 06:00
PHST- 2016/10/24 00:00 [received]
PHST- 2017/02/06 00:00 [revised]
PHST- 2017/02/13 00:00 [accepted]
PHST- 2017/04/04 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2018/06/16 06:00 [medline]
AID - S0003-2670(17)30251-9 [pii]
AID - 10.1016/j.aca.2017.02.034 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2017 May 1;965:54-62. doi: 10.1016/j.aca.2017.02.034. Epub 2017 
      Mar 6.