PMID- 28366719
OWN - NLM
STAT- MEDLINE
DCOM- 20170629
LR  - 20201209
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 180
DP  - 2017 Jul 1
TI  - Minocycline restores cognitive-relative altered proteins in young bile 
      duct-ligated rat prefrontal cortex.
PG  - 75-82
LID - S0024-3205(17)30117-0 [pii]
LID - 10.1016/j.lfs.2017.03.023 [doi]
AB  - AIMS: Bile duct ligation (BDL) model is used to study hepatic encephalopathy 
      accompanied by cognitive impairment. We employed the proteomic analysis approach 
      to evaluate cognition-related proteins in the prefrontal cortex of young BDL rats 
      and analyzed the effect of minocycline on these proteins and spatial memory. MAIN 
      METHODS: BDL was induced in young rats at postnatal day 17. Minocycline as a 
      slow-release pellet was implanted into the peritoneum. Morris water maze test and 
      two-dimensional liquid chromatography-tandem mass spectrometry were used to 
      evaluate spatial memory and prefrontal cortex protein expression, respectively. 
      We used 2D/LC-MS/MS to analyze for affected proteins in the prefrontal cortex of 
      young BDL rats. Results were verified with Western blotting, 
      immunohistochemistry, and quantitative real-time PCR. The effect of minocycline 
      in BDL rats was assessed. KEY FINDINGS: BDL induced spatial deficits, while 
      minocycline rescued it. Collapsin response mediator protein 2 (CRMP2) and 
      manganese-dependent superoxide dismutase (MnSOD) were upregulated and nucleoside 
      diphosphate kinase B (NME2) was downregulated in young BDL rats. BDL rats 
      exhibited decreased levels of brain-derived neurotrophic factor (BDNF) mRNA as 
      compared with those by the control. However, minocycline treatment restored CRMP2 
      and NME2 protein expression, BDNF mRNA level, and MnSOD activity to control 
      levels. SIGNIFICANCE: We demonstrated that BDL altered the expression of CRMP2, 
      NME2, MnSOD, and BDNF in the prefrontal cortex of young BDL rats. However, 
      minocycline treatment restored the expression of the affected mediators that are 
      implicated in cognition.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Li, Shih-Wen
AU  - Li SW
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan; Department of Medical 
      Research, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
FAU - Chen, Yu-Chieh
AU  - Chen YC
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan.
FAU - Sheen, Jiunn-Ming
AU  - Sheen JM
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan; Department of Medical 
      Research, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.
FAU - Hsu, Mei-Hsin
AU  - Hsu MH
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan.
FAU - Tain, You-Lin
AU  - Tain YL
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan.
FAU - Chang, Kow-Aung
AU  - Chang KA
AD  - Department of Anesthesiolgy, Kaohsiung Chang Gung Memorial Hospital and Chang 
      Gung University College of Medicine, Kaohsiung, Taiwan.
FAU - Huang, Li-Tung
AU  - Huang LT
AD  - Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung 
      University College of Medicine, Kaohsiung, Taiwan; Department of Traditional 
      Medicine, Chang Gung University, Linkow, Taiwan. Electronic address: 
      huangli@cgmh.org.tw.
LA  - eng
PT  - Journal Article
DEP - 20170331
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (NM23 Nucleoside Diphosphate Kinases)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (collapsin response mediator protein-2)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - FYY3R43WGO (Minocycline)
SB  - IM
MH  - Animals
MH  - Bile Ducts/pathology
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/drug effects
MH  - Chromatography, Liquid
MH  - Cognition Disorders/*drug therapy/etiology
MH  - Delayed-Action Preparations
MH  - Disease Models, Animal
MH  - Hepatic Encephalopathy/complications/*drug therapy
MH  - Intercellular Signaling Peptides and Proteins
MH  - Male
MH  - Maze Learning/drug effects
MH  - Minocycline/administration & dosage/*pharmacology
MH  - NM23 Nucleoside Diphosphate Kinases/genetics
MH  - Nerve Tissue Proteins/genetics
MH  - Prefrontal Cortex/*drug effects
MH  - Proteomics/methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Spatial Memory/*drug effects
MH  - Superoxide Dismutase/metabolism
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Bile duct ligation
OT  - Cognition
OT  - Collapsin response mediator protein 2 (CRMP2)
OT  - Manganese-dependent superoxide dismutase (MnSOD)
OT  - Minocycline
OT  - Nucleoside diphosphate kinase B (NME2)
OT  - Proteomic
OT  - Spatial memory
EDAT- 2017/04/04 06:00
MHDA- 2017/07/01 06:00
CRDT- 2017/04/04 06:00
PHST- 2017/01/04 00:00 [received]
PHST- 2017/03/24 00:00 [revised]
PHST- 2017/03/29 00:00 [accepted]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
PHST- 2017/04/04 06:00 [entrez]
AID - S0024-3205(17)30117-0 [pii]
AID - 10.1016/j.lfs.2017.03.023 [doi]
PST - ppublish
SO  - Life Sci. 2017 Jul 1;180:75-82. doi: 10.1016/j.lfs.2017.03.023. Epub 2017 Mar 31.