PMID- 28366985
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 0912-0009 (Print)
IS  - 1880-5086 (Electronic)
IS  - 0912-0009 (Linking)
VI  - 60
IP  - 2
DP  - 2017 Mar
TI  - Prosaposin knockdown in Caco-2 cells decreases cellular levels of coenzyme Q10 
      and ATP, and results in the loss of tight junction barriers.
PG  - 81-85
LID - 10.3164/jcbn.16-32 [doi]
AB  - Coenzyme Q10 (CoQ10) is a key component of the mitochondrial electron transfer 
      chain and is one of the most important antioxidants. We previously found that 
      glycoprotein prosaposin (Psap) binds CoQ10 in human cells. Although Psap is 
      expressed in the intestines, its role in the gastrointestinal tract is not clear. 
      To elucidate the role of Psap in the intestines, we established Psap knockdown 
      (KD) Caco-2 cells, which are an intestinal epithelial cell line. Cellular CoQ10 
      levels decreased significantly in Psap KD Caco-2 cells as compared to parental 
      Caco-2 cells. Cellular ATP levels also decreased significantly in Psap KD Caco-2 
      cells as compared to parental Caco-2 cells. Lower ATP levels in the intestines 
      have been reported to result in the failure of tight junction formation. As 
      expected, Psap KD Caco-2 monolayers did not produce transepithelial electrical 
      resistance, while parental Caco-2 monolayers did. Moreover, a fluorescent dye, 
      lucifer yellow, leaked out through Psap KD Caco-2 monolayers, whereas it did not 
      through parental Caco-2 monolayers. These results indicate that Psap is essential 
      to maintain cellular levels of CoQ10 and ATP, and consequently to form tight 
      junctions in the gastrointestinal tract.
FAU - Kashiba, Misato
AU  - Kashiba M
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji-shi, Tokyo 192-0982, Japan.
FAU - Terashima, Masayuki
AU  - Terashima M
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji-shi, Tokyo 192-0982, Japan.
FAU - Sagawa, Tomofumi
AU  - Sagawa T
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji-shi, Tokyo 192-0982, Japan.
FAU - Yoshimura, Shinichi
AU  - Yoshimura S
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji-shi, Tokyo 192-0982, Japan.
FAU - Yamamoto, Yorihiro
AU  - Yamamoto Y
AD  - School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 
      Katakura-cho, Hachioji-shi, Tokyo 192-0982, Japan.
LA  - eng
PT  - Journal Article
DEP - 20161206
PL  - Japan
TA  - J Clin Biochem Nutr
JT  - Journal of clinical biochemistry and nutrition
JID - 8700907
PMC - PMC5370525
OTO - NOTNLM
OT  - Caco-2
OT  - coenzyme Q10
OT  - prosaposin
OT  - transepithelial electrical resistance
COIS- We have not received any financial support or other benefits from commercial 
      sources for the work reported in the manuscript. None of the authors have 
      financial interests that could create a potential conflict of interest or the 
      appearance of a conflict of interest with regard to this work.
EDAT- 2017/04/04 06:00
MHDA- 2017/04/04 06:01
PMCR- 2017/03/01
CRDT- 2017/04/04 06:00
PHST- 2016/03/24 00:00 [received]
PHST- 2016/08/02 00:00 [accepted]
PHST- 2017/04/04 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/04/04 06:01 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - jcbn16-32 [pii]
AID - 10.3164/jcbn.16-32 [doi]
PST - ppublish
SO  - J Clin Biochem Nutr. 2017 Mar;60(2):81-85. doi: 10.3164/jcbn.16-32. Epub 2016 Dec 
      6.