PMID- 28367020
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 0974-2727 (Print)
IS  - 0974-7826 (Electronic)
IS  - 0974-2727 (Linking)
VI  - 9
IP  - 2
DP  - 2017 Apr-Jun
TI  - Comparison of four phenotypic methods for detection of 
      metallo-beta-lactamase-producing Gram-negative bacteria in rural teaching hospital.
PG  - 81-83
LID - 10.4103/0974-2727.199624 [doi]
AB  - CONTEXT: Metallo-beta-lactamase (MBL)-producing bacteria lead to resistance to 
      carbapenem an antibiotic that used as the last resort for treatment of 
      multidrug-resistant bacteria, extended spectrum beta-lactamases, and AmpC 
      beta-lactamase-producing Gram-negative bacteria (GNB). The emergence of 
      MBL-producing GNB is challenge to microbiology laboratories because there are no 
      standardized guidelines available to detect them. The aim of this study was to 
      compare four phenotypic methods to detect MBL production in GNB and to determine 
      antibiotic sensitivity of MBL-producing isolates. MATERIALS AND METHODS: A total 
      of 107 clinical isolates of GNB were tested for MBL production. Imipenem 
      (IPM)-resistant GNB were taken as positive for MBL screening. MBL detection was 
      done using ethylene diamine tetra acetic acid (EDTA) as MBL inhibitor. Four 
      phenotypic methods were evaluated: (1) Combined disk synergy test (CDST) with 
      0.5M EDTA (CDST-0.5 M EDTA), (2) CDST with 0.1 M EDTA (CDST-0.1 M EDTA), (3) 
      double-disk synergy test (DDST) with 0.5M EDTA (DDST-0.5 M EDTA), and (4) DDST 
      with 0.1 M EDTA (DDST-0.1 M EDTA). RESULTS: Out of 107 GNB, 30 were resistant to 
      IPM considered as screening positive. Out of 30, 21 (70%) isolates were MBL 
      positive by CDST-0.1 M EDTA, 19 (63.33%) by CDST-0.5M EDTA, 17 (56.67%) by 
      DDST-0.1 M EDTA, and 16 (53.33%) by DDST-0.5M EDTA. All MBL-producing 
      Gram-negative Bacilli were resistant to ampicillin/sulbactam. Polymyxin B was 
      found to be the most sensitive drug. CONCLUSION: CDST-0.1 M EDTA is the most 
      sensitive method MBL detection. The detection of MBL-producing GNB is very 
      important to control spread of the resistance.
FAU - Panchal, Chinjal A
AU  - Panchal CA
AD  - Department of Microbiology, GMERS Medical College, Gotri, Gujarat, India.
FAU - Oza, Sweta Sunil
AU  - Oza SS
AD  - Department of Microbiology, CU Shah Medical College and Hospital, Surendranagar, 
      Gujarat, India.
FAU - Mehta, Sanjay J
AU  - Mehta SJ
AD  - Department of Microbiology, CU Shah Medical College and Hospital, Surendranagar, 
      Gujarat, India.
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Lab Physicians
JT  - Journal of laboratory physicians
JID - 101551511
PMC - PMC5320885
OTO - NOTNLM
OT  - Carbapenem
OT  - Gram-negative bacteria
OT  - combined disk test
OT  - double-disk test
OT  - ethylene diamine tetra acetic acid
OT  - imipenem
OT  - metallo-beta-lactamase
COIS- There are no conflicts of interest.
EDAT- 2017/04/04 06:00
MHDA- 2017/04/04 06:01
PMCR- 2017/04/01
CRDT- 2017/04/04 06:00
PHST- 2017/04/04 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/04/04 06:01 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - JLP-9-81 [pii]
AID - 10.4103/0974-2727.199624 [doi]
PST - ppublish
SO  - J Lab Physicians. 2017 Apr-Jun;9(2):81-83. doi: 10.4103/0974-2727.199624.