PMID- 28367105
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221109
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 17
DP  - 2017
TI  - MYCN amplification predicts poor prognosis based on interphase fluorescence in 
      situ hybridization analysis of bone marrow cells in bone marrow metastases of 
      neuroblastoma.
PG  - 43
LID - 10.1186/s12935-017-0412-z [doi]
LID - 43
AB  - BACKGROUND: MYCN gene amplification is related to risk stratification. Therefore 
      it is important to identify accurately the level of the MYCN gene as early as 
      possible in neuroblastoma (NB); however, for patients with bone marrow (BM) 
      metastasis who need chemotherapy before surgery, timely detection of the MYCN 
      gene is not possible due to the unavailability of primary tumors. METHODS: MYCN 
      gene status was evaluated in 81 BM metastases of NB by interphase fluorescence in 
      situ hybridization (FISH) analysis of BM cells. The clinicobiological 
      characteristics and prognostic impact of MYCN amplification in NB metastatic to 
      BM were analyzed. RESULTS: MYCN amplification was found in 16% of patients with 
      metastases, and the results were consistent with the primary tumors detected by 
      pathological tissue FISH. MYCN amplification was associated with age, lactate 
      dehydrogenase (LDH) levels and prognosis (P = 0.038, P < 0.001, P = 0.026). 
      Clinical outcome was poorer in patients with MYCN amplification than in those 
      without amplification (3-year EFS 28.8 +/- 13.1 vs. 69.7 +/- 5.7%, P = 0.005; 3-year 
      OS 41.5 +/- 14.7 vs. 76.7 +/- 5.5%, P = 0.005). CONCLUSIONS: MYCN amplification 
      predicts a poor outcome in NB metastatic to BM, and interphase FISH of bone 
      marrow cells provides a timely direct and valid method to evaluate the MYCN gene 
      status.
FAU - Yue, Zhi-Xia
AU  - Yue ZX
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Huang, Cheng
AU  - Huang C
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Gao, Chao
AU  - Gao C
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Xing, Tian-Yu
AU  - Xing TY
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Liu, Shu-Guang
AU  - Liu SG
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Li, Xing-Jun
AU  - Li XJ
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Zhao, Qian
AU  - Zhao Q
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Wang, Xi-Si
AU  - Wang XS
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Zhao, Wen
AU  - Zhao W
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Jin, Mei
AU  - Jin M
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
FAU - Ma, Xiao-Li
AU  - Ma XL
AD  - Beijing Key Laboratory of Pediatric Hematology Oncology, National Key Discipline 
      of Pediatrics, Ministry of Education, MOE Key Laboratory of Major Diseases in 
      Children, Hematology Oncology Center, Beijing Children's Hospital, Capital 
      Medical University, 56 Nanlishi Road, Beijing, 100045 China. GRID: grid.24696.3f
LA  - eng
PT  - Journal Article
DEP - 20170331
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC5374581
OTO - NOTNLM
OT  - Bone marrow metastasis
OT  - Fluorescence in situ hybridization
OT  - MYCN amplification
OT  - Neuroblastoma
EDAT- 2017/04/04 06:00
MHDA- 2017/04/04 06:01
PMCR- 2017/03/31
CRDT- 2017/04/04 06:00
PHST- 2016/08/10 00:00 [received]
PHST- 2017/03/28 00:00 [accepted]
PHST- 2017/04/04 06:00 [entrez]
PHST- 2017/04/04 06:00 [pubmed]
PHST- 2017/04/04 06:01 [medline]
PHST- 2017/03/31 00:00 [pmc-release]
AID - 412 [pii]
AID - 10.1186/s12935-017-0412-z [doi]
PST - epublish
SO  - Cancer Cell Int. 2017 Mar 31;17:43. doi: 10.1186/s12935-017-0412-z. eCollection 
      2017.