PMID- 28372836
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20240109
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1497
DP  - 2017 May 12
TI  - Macroalga Padina pavonica water extracts obtained by pressurized liquid 
      extraction and microwave-assisted extraction inhibit hyaluronidase activity as 
      shown by capillary electrophoresis.
PG  - 19-27
LID - S0021-9673(17)30412-0 [pii]
LID - 10.1016/j.chroma.2017.03.033 [doi]
AB  - Hyaluronidase degrades hyaluronic acid, the principal component of the 
      extracellular matrix. Inhibition of this enzyme is thus expected to hinder skin 
      aging. Brown alga Padina pavonica activity toward hyaluronidase was evaluated 
      using capillary electrophoresis (CE)-based enzymatic assays. This green technique 
      allows evaluation of the biological activity of the natural material in an 
      economic manner. Pressurized liquid extraction (PLE), microwave assisted 
      extraction (MAE), supercritical fluid extraction and electroporation extraction 
      techniques were used. Extraction conditions were optimized to obtain cosmetically 
      acceptable Padina pavonica extracts with the best inhibition activity. CE-based 
      assays were conducted using only a few nanoliters of reactants, a capillary of 
      60cm total length and of 50mum internal diameter, +20kV voltage for separation in 
      50mM ammonium acetate buffer (pH 9.0) and 200nm wavelength for detection. The 
      reaction mixture was incubated for 1h and CE analysis time was about 11min. A 
      novel online CE-assay using transverse diffusion of laminar flow profiles for 
      in-capillary reactant mixing allowed efficient monitoring of hyaluronidase 
      kinetics with K(m) and V(max) equal to 0.46+/-0.04mgmL(-1) and 137.1+/-0.3nMs(-1) 
      (r(2)=0.99; n=3), respectively. These values compared well with literature, which 
      validates the assay. Water extracts obtained by PLE (60 degrees C; 2 cycles) and MAE 
      (60 degrees C; 1000W; 2min) presented the highest anti-hyaluronidase activity. The half 
      maximal effective concentration (IC(50)) of water PLE extract was 
      0.04+/-0.01mgmL(-1) (r(2)=0.99; n=3). This value is comparable to the one obtained 
      for Einsenia bicyclis phlorotannin fractions (IC(50)=0.03mgmL(-1)), which makes 
      Padina pavonica bioactivity very promising.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Fayad, Syntia
AU  - Fayad S
AD  - Institut de Chimie Organique et Analytique (ICOA), Universite d'Orleans - CNRS FR 
      2708 - UMR 7311, Orleans, France.
FAU - Nehme, Reine
AU  - Nehme R
AD  - Institut de Chimie Organique et Analytique (ICOA), Universite d'Orleans - CNRS FR 
      2708 - UMR 7311, Orleans, France. Electronic address: 
      reine.nehme@univ-orleans.fr.
FAU - Tannoury, Mona
AU  - Tannoury M
AD  - Faculty of Science II, Department of Biology, Lebanese University, 90656 Jdeidet 
      El Metn, Fanar, Lebanon.
FAU - Lesellier, Eric
AU  - Lesellier E
AD  - Institut de Chimie Organique et Analytique (ICOA), Universite d'Orleans - CNRS FR 
      2708 - UMR 7311, Orleans, France.
FAU - Pichon, Chantal
AU  - Pichon C
AD  - Centre de Biophysique moleculaire, CNRS UPR4301 and Universite d'Orleans, France.
FAU - Morin, Philippe
AU  - Morin P
AD  - Institut de Chimie Organique et Analytique (ICOA), Universite d'Orleans - CNRS FR 
      2708 - UMR 7311, Orleans, France.
LA  - eng
PT  - Journal Article
DEP - 20170320
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 059QF0KO0R (Water)
RN  - EC 3.2.1.35 (Hyaluronoglucosaminidase)
SB  - IM
MH  - Chromatography, Supercritical Fluid
MH  - Diffusion
MH  - Electrophoresis, Capillary/*methods/standards
MH  - Electroporation
MH  - Hyaluronoglucosaminidase/*antagonists & inhibitors
MH  - Inhibitory Concentration 50
MH  - Kinetics
MH  - Liquid-Liquid Extraction/*methods
MH  - *Microwaves
MH  - Phaeophyceae/*chemistry
MH  - *Pressure
MH  - Reproducibility of Results
MH  - Seaweed/*chemistry
MH  - Water/*chemistry
OTO - NOTNLM
OT  - Algae
OT  - Capillary electrophoresis
OT  - Electroporation
OT  - Enzyme kinetics
OT  - Hyaluronidase
EDAT- 2017/04/05 06:00
MHDA- 2017/05/10 06:00
CRDT- 2017/04/05 06:00
PHST- 2016/11/30 00:00 [received]
PHST- 2017/03/16 00:00 [revised]
PHST- 2017/03/17 00:00 [accepted]
PHST- 2017/04/05 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2017/04/05 06:00 [entrez]
AID - S0021-9673(17)30412-0 [pii]
AID - 10.1016/j.chroma.2017.03.033 [doi]
PST - ppublish
SO  - J Chromatogr A. 2017 May 12;1497:19-27. doi: 10.1016/j.chroma.2017.03.033. Epub 
      2017 Mar 20.