PMID- 28374250
OWN - NLM
STAT- MEDLINE
DCOM- 20180207
LR  - 20180516
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1581
DP  - 2017
TI  - Human Rhinovirus-A1 as an Expression Vector.
PG  - 181-201
LID - 10.1007/978-1-4939-6869-5_11 [doi]
AB  - Expression vectors that are based on live human rhinoviruses (HRVs) are 
      attractive, yet often overlooked in vaccine development due to their limited 
      capacity for foreign gene inserts and poor genetic stability. This chapter 
      describes a novel methodology to engineer a replication-competent genetically 
      stable recombinant HRV (rHRV) without affecting viral replication capability. We 
      have previously used these methods to generate live, genetically stable 
      recombinant HRVs encoding HIV Gag and Tat proteins (rHRV-Gag-Tat), a potential 
      mucosally targeted HIV vaccine.
FAU - Tomusange, Khamis
AU  - Tomusange K
AD  - Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of 
      Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia. 
      khamis.tomusange@adelaide.edu.au.
FAU - Wijesundara, Danushka
AU  - Wijesundara D
AD  - Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of 
      Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
FAU - Gowans, Eric James
AU  - Gowans EJ
AD  - Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of 
      Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
FAU - Grubor-Bauk, Branka
AU  - Grubor-Bauk B
AD  - Virology Laboratory, Basil Hetzel Institute, Discipline of Surgery, University of 
      Adelaide, Woodville Road, Woodville South, Adelaide, SA, 5011, Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (gag Gene Products, Human Immunodeficiency Virus)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - Rhinovirus A
SB  - IM
MH  - Enterovirus/genetics/*physiology
MH  - Genetic Vectors
MH  - HeLa Cells
MH  - Humans
MH  - Virus Replication
MH  - gag Gene Products, Human Immunodeficiency Virus/*genetics/immunology
MH  - tat Gene Products, Human Immunodeficiency Virus/*genetics/immunology
OTO - NOTNLM
OT  - HIV-Gag and HIV-Tat
OT  - HRV-A1
OT  - Live vaccine vector
EDAT- 2017/04/05 06:00
MHDA- 2018/02/08 06:00
CRDT- 2017/04/05 06:00
PHST- 2017/04/05 06:00 [entrez]
PHST- 2017/04/05 06:00 [pubmed]
PHST- 2018/02/08 06:00 [medline]
AID - 10.1007/978-1-4939-6869-5_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1581:181-201. doi: 10.1007/978-1-4939-6869-5_11.