PMID- 28376277
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20171116
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 85
IP  - 6
DP  - 2017 Jun
TI  - Dendritic Cells from Rheumatoid Arthritis Patient Peripheral Blood Induce Th17 
      Cell Differentiation via miR-363/Integrin alphav/TGF-beta Axis.
PG  - 441-449
LID - 10.1111/sji.12550 [doi]
AB  - Dendritic cells (DCs) are critical regulators of immune responses. This study was 
      to observe the effect of DCs from peripheral blood on the differentiation of Th17 
      in patients with rheumatoid arthritis (RA). Peripheral blood samples were 
      collected from 30 patients with RA and 20 healthy controls, respectively. Flow 
      cytometry results showed that in contrast to Treg cells, the proportion of Th17 
      cells in T cells and the Th17/Treg ratio were both increased in patients with RA. 
      The RT-PCR results showed that Foxp3、ROR gammat and miR-363 expression in PBMC of 
      patients with RA were reduced, but the ITGAV expression was increased, which was 
      negatively related to miR-363 expression. IL-17, TGF-beta and IL-6 levels detected 
      by ELISA were increased in peripheral blood serum of patients with RA. Moreover, 
      we noted that the CD11C(+) alphanu(+) /CD11C(+) DCs ratio was obvious increased in 
      patients with RA and has positive correlation to the Th17/Treg ratio. In 
      cocultured system, Th17 cell differentiation was significantly inhibited in the 
      presence of ITGF-beta suggesting that Th17 cell differentiation was controlled by 
      active TGF-beta (aTGF-beta). After DCs transfecting with miR-363 mimics and cocultured 
      with T cells, Th17 cell number, IL-17 level and ROR-gammat expression were 
      significantly reduced in the presence of latent TGF-beta (ITGF-beta). In addition, the 
      integrin alphav protein expression was both reduced in the presence of aTGF-beta or 
      ITGF-beta. These data demonstrated that DCs induced Th17 cell differentiation 
      through miR-363/Integrin alphav/TGF-beta pathway in patients with RA.
CI  - (c) 2017 The Foundation for the Scandinavian Journal of Immunology.
FAU - Pan, F
AU  - Pan F
AUID- ORCID: 0000-0002-2690-3721
FAU - Xiang, H
AU  - Xiang H
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Yan, J
AU  - Yan J
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Hong, L
AU  - Hong L
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Zhang, L
AU  - Zhang L
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Liu, Y
AU  - Liu Y
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Feng, X
AU  - Feng X
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
FAU - Cai, C
AU  - Cai C
AD  - The Affiliated Hospotal of Hangzhou Normal University, Hangzhou, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (CD11c Antigen)
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Integrin alphaV)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN363 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/blood/*immunology
MH  - Blotting, Western
MH  - CD11c Antigen/immunology/metabolism
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Forkhead Transcription Factors/genetics/immunology/metabolism
MH  - Gene Expression/immunology
MH  - Humans
MH  - Integrin alphaV/*immunology/metabolism
MH  - Interleukin-17/blood/immunology/metabolism
MH  - Interleukin-6/blood/immunology/metabolism
MH  - Male
MH  - MicroRNAs/genetics/*immunology/metabolism
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics/immunology
MH  - T-Lymphocytes/immunology/metabolism
MH  - T-Lymphocytes, Regulatory/immunology/metabolism
MH  - Th17 Cells/*immunology
MH  - Transforming Growth Factor beta/blood/*immunology/metabolism
EDAT- 2017/04/05 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/04/05 06:00
PHST- 2016/11/22 00:00 [received]
PHST- 2017/02/22 00:00 [revised]
PHST- 2017/03/13 00:00 [accepted]
PHST- 2017/04/05 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2017/04/05 06:00 [entrez]
AID - 10.1111/sji.12550 [doi]
PST - ppublish
SO  - Scand J Immunol. 2017 Jun;85(6):441-449. doi: 10.1111/sji.12550.