PMID- 28379411
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20190816
IS  - 2049-632X (Electronic)
IS  - 2049-632X (Linking)
VI  - 75
IP  - 5
DP  - 2017 Jul 31
TI  - Comparison of the inflammatory response of brain microvascular and peripheral 
      endothelial cells following infection with Neisseria meningitidis.
LID - 10.1093/femspd/ftx038 [doi]
AB  - The interaction of Neisseria meningitidis with both peripheral and brain 
      endothelial cells is a critical event in the development of invasive 
      meningococcal disease. In this study, we used in vitro models based on human 
      brain microvascular endothelial cells (HBMEC), and peripheral endothelial 
      EA.hy926 cells, to investigate their roles in the inflammatory response towards 
      meningococcal infection. Both cell lines were infected with two pathogenic N. 
      meningitidis isolates and secretion of the cytokine interleukin-6 (IL-6), the CXC 
      chemokine IL-8 and the monocyte chemoattractant protein-1 (MCP-1) were estimated 
      by ELISA. Neisseria meningitidis was able to stimulate the production of IL-6 and 
      IL-8 by HBMEC and EA.hy926 cells in a time- and concentration-dependent manner. 
      Interestingly, HBMEC released significant higher amounts of IL-6 and IL-8. 
      Moreover, we observed that heat-killed bacteria stimulated high levels of IL-8. 
      In addition, capsule expression had an inhibitory effect on IL-8 release. We 
      extended our study and included serogroup C strains belonging to sequence type 11 
      clonal complex (cc) from a recent outbreak in France, as well as isolates 
      belonging to the hypervirulent clonal complexes cc8, cc18, cc32 and cc269 and 
      analyzed their ability to induce the secretion of IL-8 from both cell lines. 
      Although individual variations were observed among different isolates, no clear 
      correlations were observed between strain origin, clinical presentation and IL-8 
      levels.
CI  - (c) FEMS 2017. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Dick, Julia
AU  - Dick J
AD  - Institute of Hygiene and Microbiology, Julius-Maximilians University, 97080 
      Wurzburg, Germany.
FAU - Hebling, Sabrina
AU  - Hebling S
AD  - Institute of Hygiene and Microbiology, Julius-Maximilians University, 97080 
      Wurzburg, Germany.
FAU - Becam, Jerome
AU  - Becam J
AD  - Institute of Hygiene and Microbiology, Julius-Maximilians University, 97080 
      Wurzburg, Germany.
FAU - Taha, Muhamed-Kheir
AU  - Taha MK
AD  - Institut Pasteur, Unit of Invasive Bacterial Infections, Paris 75015, France.
FAU - Schubert-Unkmeir, Alexandra
AU  - Schubert-Unkmeir A
AD  - Institute of Hygiene and Microbiology, Julius-Maximilians University, 97080 
      Wurzburg, Germany.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Pathog Dis
JT  - Pathogens and disease
JID - 101595366
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/*metabolism
MH  - Disease Outbreaks
MH  - Endothelial Cells/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - France/epidemiology
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Interleukin-6/analysis/*metabolism
MH  - Interleukin-8/analysis/*metabolism
MH  - Meningitis, Meningococcal/epidemiology/microbiology
MH  - Neisseria meningitidis/*immunology/isolation & purification
OTO - NOTNLM
OT  - IL-6
OT  - IL-8
OT  - MCP-1
OT  - Neisseria meningitidis
OT  - brain and peripheral endothelial cells
OT  - hypervirulent clonal complexes
EDAT- 2017/04/06 06:00
MHDA- 2018/02/24 06:00
CRDT- 2017/04/06 06:00
PHST- 2016/12/09 00:00 [received]
PHST- 2017/03/29 00:00 [accepted]
PHST- 2017/04/06 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2017/04/06 06:00 [entrez]
AID - 3098218 [pii]
AID - 10.1093/femspd/ftx038 [doi]
PST - ppublish
SO  - Pathog Dis. 2017 Jul 31;75(5). doi: 10.1093/femspd/ftx038.