PMID- 28382020
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - H9N2 Avian Influenza Virus Protein PB1 Enhances the Immune Responses of Bone 
      Marrow-Derived Dendritic Cells by Down-Regulating miR375.
PG  - 287
LID - 10.3389/fmicb.2017.00287 [doi]
LID - 287
AB  - Polymerase basic protein 1 (PB1), the catalytic core of the influenza A virus RNA 
      polymerase complex, is essential for viral transcription and replication. 
      Dendritic cells (DCs) possess important antigen presenting ability and a crucial 
      role in recognizing and clearing virus. MicroRNA (miRNA) influence the 
      development of DCs and their ability to present antigens as well as the ability 
      of avian influenza virus (AIV) to infect host cells and replicate. Here, we 
      studied the molecular mechanism underlying the miRNA-mediated regulation of 
      immune function in mouse DCs. We first screened for and verified the induction of 
      miRNAs in DCs after PB1 transfection. Results showed that the viral protein PB1 
      down-regulated the expression of miR375, miR146, miR339, and miR679 in DCs, 
      consistent with the results of H9N2 virus treatment; however, the expression of 
      miR222 and miR499, also reduced in the presence of PB1, was in contrast to the 
      results of H9N2 virus treatment. Our results suggest that PB1 enhanced the 
      ability of DCs to present antigens, activate lymphocytes, and secrete cytokines, 
      while miR375 over-expression repressed activation of DC maturation. Nevertheless, 
      PB1 could not promote DC maturation once miR375 was inhibited. Finally, we 
      revealed that PB1 inhibited the P-Jnk/Jnk signaling pathway, but activated the 
      p-Erk/Erk signaling pathway. While inhibition of miR375 -activated the p-Erk/Erk 
      and p-p38/p38 signaling pathway, but repressed the P-Jnk/Jnk signaling pathway. 
      Taken together, results of our studies shed new light on the roles and mechanisms 
      of PB1 and miR375 in regulating DC function and suggest new strategies for 
      combating AIV.
FAU - Lin, Jian
AU  - Lin J
AD  - Department of Zoology, College of Life Science, Nanjing Agricultural University 
      Jiangsu, China.
FAU - Xia, Jing
AU  - Xia J
AD  - Department of Zoology, College of Life Science, Nanjing Agricultural University 
      Jiangsu, China.
FAU - Tu, Chong Z
AU  - Tu CZ
AD  - Department of Histoembryology, College of Veterinary Medicine, Nanjing 
      Agricultural University Jiangsu, China.
FAU - Zhang, Ke Y
AU  - Zhang KY
AD  - Department of Zoology, College of Life Science, Nanjing Agricultural University 
      Jiangsu, China.
FAU - Zeng, Yan
AU  - Zeng Y
AD  - Department of Zoology, College of Life Science, Nanjing Agricultural University 
      Jiangsu, China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Department of Zoology, College of Life Science, Nanjing Agricultural University 
      Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20170322
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5360757
OTO - NOTNLM
OT  - H9N2 AIV
OT  - PB1
OT  - dendritic cells
OT  - immune regulation
OT  - miRNA
EDAT- 2017/04/07 06:00
MHDA- 2017/04/07 06:01
PMCR- 2017/03/22
CRDT- 2017/04/07 06:00
PHST- 2016/09/26 00:00 [received]
PHST- 2017/02/13 00:00 [accepted]
PHST- 2017/04/07 06:00 [entrez]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2017/04/07 06:01 [medline]
PHST- 2017/03/22 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.00287 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Mar 22;8:287. doi: 10.3389/fmicb.2017.00287. eCollection 
      2017.