PMID- 28382156
OWN - NLM
STAT- MEDLINE
DCOM- 20171024
LR  - 20221123
IS  - 1838-7640 (Electronic)
IS  - 1838-7640 (Linking)
VI  - 7
IP  - 4
DP  - 2017
TI  - Theranostic Nucleic Acid Binding Nanoprobe Exerts Anti-inflammatory and 
      Cytoprotective Effects in Ischemic Injury.
PG  - 814-825
LID - 10.7150/thno.17366 [doi]
AB  - Extracellular nucleic acids are proinflammatory molecules that have been 
      implicated in a diverse range of diseases. We report here the development of a 
      multivalent nucleic acid scavenging nanoprobe, where the fluorochrome thiazole 
      orange (TO) is conjugated to a polymeric 40 kDa dextran carrier. Dextran-TO 
      (Dex-TO) has nanomolar affinity for mammalian and bacterial nucleic acids and 
      attenuates the production of inflammatory cytokines from activated macrophages 
      exposed to DNA and RNA. Mice with myocardial ischemia reperfusion that were 
      treated with Dex-TO showed a decrease in myocardial macrophage infiltration at 24 
      hours (p<0.05) and a decrease in infarct size (18% +/- 9%, p<0.01) on day 7. Dex-TO 
      allows sites of injury to be identified with fluorescence imaging, while 
      simultaneously exerting an anti-inflammatory and cytoprotective effect. Dex-TO 
      could be of significant diagnostic and therapeutic (theranostic) utility in a 
      broad range of conditions including ischemia, trauma, burns, sepsis and 
      autoimmune disease.
FAU - Chen, Howard H
AU  - Chen HH
AD  - Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard 
      Medical School, Boston MA;; Cardiovascular Research Center, Massachusetts General 
      Hospital, Harvard Medical School, Boston MA.
FAU - Yuan, Hushan
AU  - Yuan H
AD  - Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 
      Boston MA (H.C.: currently School of Materials Science and Engineering, Chonnam 
      National University, Gwangju, South Korea).
FAU - Cho, Hoonsung
AU  - Cho H
AD  - Department of Radiology, Massachusetts General Hospital, Harvard Medical School, 
      Boston MA (H.C.: currently School of Materials Science and Engineering, Chonnam 
      National University, Gwangju, South Korea).
FAU - Feng, Yan
AU  - Feng Y
AD  - Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General 
      Hospital, Harvard Medical School, Boston MA (currently Department of 
      Anesthesiology, University of Maryland School of Medicine, Baltimore MD).
FAU - Ngoy, Soeun
AU  - Ngoy S
AD  - Division of Genetics and Division of Cardiovascular Medicine, Department of 
      Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston MA.
FAU - Kumar, Anand T N
AU  - Kumar AT
AD  - Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard 
      Medical School, Boston MA.
FAU - Liao, Ronglih
AU  - Liao R
AD  - Division of Genetics and Division of Cardiovascular Medicine, Department of 
      Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston MA.
FAU - Chao, Wei
AU  - Chao W
AD  - Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General 
      Hospital, Harvard Medical School, Boston MA (currently Department of 
      Anesthesiology, University of Maryland School of Medicine, Baltimore MD).
FAU - Josephson, Lee
AU  - Josephson L
AD  - Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard 
      Medical School, Boston MA;; Department of Radiology, Massachusetts General 
      Hospital, Harvard Medical School, Boston MA (H.C.: currently School of Materials 
      Science and Engineering, Chonnam National University, Gwangju, South Korea).
FAU - Sosnovik, David E
AU  - Sosnovik DE
AD  - Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard 
      Medical School, Boston MA;; Cardiovascular Research Center, Massachusetts General 
      Hospital, Harvard Medical School, Boston MA.
LA  - eng
GR  - R01 HL093038/HL/NHLBI NIH HHS/United States
GR  - R01 HL112831/HL/NHLBI NIH HHS/United States
GR  - R00 HL121152/HL/NHLBI NIH HHS/United States
GR  - R01 GM117233/GM/NIGMS NIH HHS/United States
GR  - K99 HL121152/HL/NHLBI NIH HHS/United States
GR  - R01 EB011996/EB/NIBIB NIH HHS/United States
GR  - S10 RR025504/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170208
PL  - Australia
TA  - Theranostics
JT  - Theranostics
JID - 101552395
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Benzothiazoles)
RN  - 0 (Dextrans)
RN  - 0 (Nucleic Acids)
RN  - 0 (Quinolines)
RN  - 107091-89-4 (thiazole orange)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage
MH  - Benzothiazoles/administration & dosage
MH  - *Cytoprotection
MH  - Dextrans/administration & dosage
MH  - Mice
MH  - Myocardial Reperfusion Injury/diagnostic imaging/*drug therapy/pathology
MH  - Nanostructures/*administration & dosage
MH  - Nucleic Acids/*metabolism
MH  - Optical Imaging
MH  - Quinolines/administration & dosage
MH  - Theranostic Nanomedicine/methods
MH  - Treatment Outcome
PMC - PMC5381246
OTO - NOTNLM
OT  - DNA
OT  - Imaging.
OT  - Inflammation
OT  - Ischemia
OT  - Nanoprobe
OT  - RNA
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2017/04/07 06:00
MHDA- 2017/10/25 06:00
PMCR- 2017/01/01
CRDT- 2017/04/07 06:00
PHST- 2016/08/27 00:00 [received]
PHST- 2016/11/25 00:00 [accepted]
PHST- 2017/04/07 06:00 [entrez]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2017/10/25 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - thnov07p0814 [pii]
AID - 10.7150/thno.17366 [doi]
PST - epublish
SO  - Theranostics. 2017 Feb 8;7(4):814-825. doi: 10.7150/thno.17366. eCollection 2017.