PMID- 28382380
OWN - NLM
STAT- MEDLINE
DCOM- 20171102
LR  - 20181202
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 49
IP  - 5
DP  - 2017 Jun
TI  - Ungulate copiparvovirus 2 in healthy and postweaning multisystemic wasting 
      syndrome-affected pigs.
PG  - 945-949
LID - 10.1007/s11250-017-1279-7 [doi]
AB  - A SYBR Green-based real-time polymerase chain reaction (qPCR) was designed to 
      detect Ungulate copiparvovirus 2, also known as porcine parvovirus 4 (PPV4). The 
      test was applied to search for PPV4 DNAemia in sera from 1- to 4-month-old pigs 
      displaying signs of postweaning multisystemic wasting syndrome (PMWS), as well as 
      in sera from healthy swine at equivalent age and in sera from older healthy 
      animals (>6 months old). High levels of PPV4 DNA were detected in PMWS-affected 
      pigs. The mean viral DNA load in PMWS-affected pigs was 5.2 x 10(7) copies/mL, 
      whereas in young healthy pigs it was 1.4 x 10(5) copies/mL (P </= 0.001). Although 
      the copy numbers were lower in younger PMWS-affected individuals, this result 
      sheds some light on the possible association between PPV4 viral load detection in 
      this group and the immune impairment caused by PMWS.
FAU - Cibulski, Samuel P
AU  - Cibulski SP
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil. spcibulski@gmail.com.
FAU - Teixeira, Thais F
AU  - Teixeira TF
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
FAU - Varela, Ana Paula M
AU  - Varela APM
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
FAU - Scheffer, Camila M
AU  - Scheffer CM
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
FAU - Santos, Helton F
AU  - Santos HF
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
FAU - Lima, Francisco E S
AU  - Lima FES
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
FAU - Roehe, Paulo M
AU  - Roehe PM
AD  - Virology Laboratory, Department of Microbiology, Immunology and Parasitology, 
      Institute of Basic Health Sciences, Federal University of Rio Grande do Sul 
      (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, 
      Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170405
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral/analysis
MH  - Parvoviridae Infections/epidemiology/*veterinary/virology
MH  - Parvovirinae/*isolation & purification
MH  - Parvovirus, Porcine/physiology
MH  - Porcine Postweaning Multisystemic Wasting Syndrome/*virology
MH  - Prevalence
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Swine Diseases/*epidemiology/virology
MH  - Viral Load/*veterinary
OTO - NOTNLM
OT  - PPV4
OT  - Ungulate copiparvovirus 2
OT  - Viral load
OT  - qPCR
EDAT- 2017/04/07 06:00
MHDA- 2017/11/03 06:00
CRDT- 2017/04/07 06:00
PHST- 2016/09/08 00:00 [received]
PHST- 2017/03/15 00:00 [accepted]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2017/11/03 06:00 [medline]
PHST- 2017/04/07 06:00 [entrez]
AID - 10.1007/s11250-017-1279-7 [pii]
AID - 10.1007/s11250-017-1279-7 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2017 Jun;49(5):945-949. doi: 10.1007/s11250-017-1279-7. 
      Epub 2017 Apr 5.