PMID- 28382439
OWN - NLM
STAT- MEDLINE
DCOM- 20170913
LR  - 20220410
IS  - 1435-702X (Electronic)
IS  - 0721-832X (Linking)
VI  - 255
IP  - 6
DP  - 2017 Jun
TI  - Plasma level of miR-93 is associated with higher risk to develop type 2 diabetic 
      retinopathy.
PG  - 1159-1166
LID - 10.1007/s00417-017-3638-5 [doi]
AB  - PURPOSE: MicroRNA-93 (miR-93) usually acts as a promoter of tumor progression in 
      several human carcinomas. It has been found distinctly high in eyes with 
      proliferative diabetic retinopathy (DR). The present study aims to investigate 
      the role of plasma miR-93 in the progression of type 2 diabetic retinopathy 
      (T2DR). METHODS: Our study subjects were made up of 140 type 2 diabetes mellitus 
      (T2DM) patients who were assigned into DR (DR patients, n = 75), NDR (non-DR 
      patients, n = 65), and control (healthy individuals, n = 127) groups. Levels of 
      fasting blood glucose (FBG), fasting plasma glucose (FPG), triglyceride (TG), 
      glycosylated hemoglobin (HbA1c), total cholesterol (TC), blood urea nitrogen 
      (BUN), creatinine (Cr), high-density lipoprotein cholesterol (HDL-C), low-density 
      lipoprotein cholesterol (LDL-C) and fasting insulin (FIsn) were detected by 
      automatic biochemical analyzer. Enzyme-linked immunosorbent assay (ELISA) was 
      performed for the levels of interleukin (IL)-1, IL-6, tumor necrosis factor 
      (TNF)-alpha and vascular endothelial growth factor (VEGF), qRT-PCR for the miR-93 
      expression in plasma, and mRNA expressions of IL-1, IL-6, TNF-alpha and VEGF; 
      receiver operating characteristic (ROC) curve for the diagnostic performance of 
      miR-93 to T2DR, Pearson correlation analysis for correlation analysis between 
      miR-93 and other indexes detected before and multivariate logistic regression 
      analyses for the risk factors for T2DR. RESULTS: The DR and NDR groups exhibited 
      elevated course of disease, and decreased levels of FBG, FPG, TG, HbA1c, TC, BUN, 
      Cr, HDL-C, FIsn, IL-1, IL-6, TNF-alpha and VEGF but declined LDL-C level as compared 
      to the control group. The course of disease was longer and the levels of FBG, 
      FPG, HbA1c, IL1, IL6 and VEGF were higher in the DR group than those in the NDR 
      group (all P < 0.05). The miR-93 expression and RNA expressions of IL-1, IL-6, 
      TNF-alpha and VEGF were higher in the DR group than those in the NDR group 
      (P < 0.05). The best cutoff for miR-93 to assess T2DR was 1.31, with a Youden 
      index of 0.63, sensitivity of 73.33%, specificity of 89.24%, and area under the 
      curve (AUC) of 0.866. Pearson correlation analysis indicated that miR-93 
      expression was positively associated with course of disease, the levels of FPG, 
      HbA1c, TNF-alpha and VEGF. T2DM patients with longer disease course, higher levels of 
      FBG, HbA1c, VEGF and miR-93 expression were risk factors for developing DR. 
      CONCLUSION: Our study demonstrates that plasma miR-93 is associated with the 
      progression of T2DR and it can sever as a diagnostic marker for T2DR.
FAU - Zou, Hui-Ling
AU  - Zou HL
AD  - Department of Endocrinology and Metabolism, Suqian People's Hospital, Nanjing 
      Drum Tower Hospital, No. 138, South Yellow River Road, Suqian, 223800, Jiangsu 
      Province, People's Republic of China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Geriatrics, Suqian People's Hospital, Nanjing Drum Tower Hospital, 
      Suqian, 223800, People's Republic of China.
FAU - Gang, Qiang
AU  - Gang Q
AD  - Department of Endocrinology and Metabolism, Suqian People's Hospital, Nanjing 
      Drum Tower Hospital, No. 138, South Yellow River Road, Suqian, 223800, Jiangsu 
      Province, People's Republic of China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Department of Endocrinology and Metabolism, Suqian People's Hospital, Nanjing 
      Drum Tower Hospital, No. 138, South Yellow River Road, Suqian, 223800, Jiangsu 
      Province, People's Republic of China.
FAU - Sun, Yu
AU  - Sun Y
AD  - Department of Endocrinology and Metabolism, Suqian People's Hospital, Nanjing 
      Drum Tower Hospital, No. 138, South Yellow River Road, Suqian, 223800, Jiangsu 
      Province, People's Republic of China. sqsunyu@126.com.
LA  - eng
PT  - Journal Article
DEP - 20170405
PL  - Germany
TA  - Graefes Arch Clin Exp Ophthalmol
JT  - Graefe's archive for clinical and experimental ophthalmology = Albrecht von 
      Graefes Archiv fur klinische und experimentelle Ophthalmologie
JID - 8205248
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (MIRN93 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers/blood
MH  - Cytokines/blood
MH  - Diabetes Mellitus, Type 2/blood/*complications
MH  - Diabetic Retinopathy/*blood/diagnosis/etiology
MH  - Disease Progression
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Male
MH  - MicroRNAs/*blood
MH  - Middle Aged
MH  - ROC Curve
MH  - Retrospective Studies
MH  - Risk Factors
OTO - NOTNLM
OT  - Diagnosis
OT  - MicroRNA-93
OT  - Sensitivity
OT  - Specificity
OT  - Type 2 diabetes mellitus
OT  - Type 2 diabetic retinopathy
EDAT- 2017/04/07 06:00
MHDA- 2017/09/14 06:00
CRDT- 2017/04/07 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/03/13 00:00 [accepted]
PHST- 2017/03/04 00:00 [revised]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2017/09/14 06:00 [medline]
PHST- 2017/04/07 06:00 [entrez]
AID - 10.1007/s00417-017-3638-5 [pii]
AID - 10.1007/s00417-017-3638-5 [doi]
PST - ppublish
SO  - Graefes Arch Clin Exp Ophthalmol. 2017 Jun;255(6):1159-1166. doi: 
      10.1007/s00417-017-3638-5. Epub 2017 Apr 5.