PMID- 2838305
OWN - NLM
STAT- MEDLINE
DCOM- 19880811
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 148
IP  - 3
DP  - 1988 Apr 13
TI  - Indications for P2-purinoceptor subtypes in guinea pig smooth muscle.
PG  - 361-70
AB  - The effects of ADP, ATP, 2-methylthio ATP, alpha,beta-methylene ADP (alpha,beta 
      meADP) and alpha,beta meATP on smooth muscle tone and the responses to transmural 
      nerve stimulation were studied in isolated longitudinal ileum muscle, in vas 
      deferens and in taenia coli of the guinea pig. The nucleotides evoked contractile 
      responses in the ileum and vas deferens preparations which were blocked by 
      p-chloromercuribenzene sulfonic acid (PCMBS) and evoked relaxation in the taenia 
      which was not affected by PCMBS. The potency order of the agonists in the ileum 
      (2-methylthio ATP was more potent than ATP which was equipotent with ADP) is 
      consistent with the suggestion that there are P2Y receptors in the taenia. The 
      antagonist pattern, including the inefficacy of reactive blue 2, instead suggests 
      a similarity between the ileum receptors and the P2X receptors in vas deferens. 
      However, alpha,beta meATP did not antagonize the contractile effect of ATP in the 
      ileum, in contrast to its effect in the vas deferens where alpha,beta meATP did 
      abolish the contractile effect of ATP. The possibilities for classification of a 
      new receptor/site of purine nucleotide action, P2S, are considered.
FAU - Wiklund, N P
AU  - Wiklund NP
AD  - Department of Physiology, Karolinska Institute, Stockholm, Sweden.
FAU - Gustafsson, L E
AU  - Gustafsson LE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Receptors, Purinergic)
RN  - 0 (Triazines)
RN  - 5DV0L8V99J (Cibacron Blue F 3GA)
RN  - 5YIN07W42H (4-Chloromercuribenzenesulfonate)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 7C0697DR9I (Atropine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 8Y164V895Y (Carbachol)
RN  - NYX13NT29D (alpha,beta-methyleneadenosine 5'-triphosphate)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - 4-Chloromercuribenzenesulfonate/pharmacology
MH  - Adenosine Diphosphate/pharmacology
MH  - Adenosine Triphosphate/analogs & derivatives/pharmacology
MH  - Animals
MH  - Atropine/pharmacology
MH  - Carbachol/pharmacology
MH  - Guinea Pigs
MH  - Indomethacin/pharmacology
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth/*analysis
MH  - Receptors, Purinergic/*analysis
MH  - Triazines
EDAT- 1988/04/13 00:00
MHDA- 1988/04/13 00:01
CRDT- 1988/04/13 00:00
PHST- 1988/04/13 00:00 [pubmed]
PHST- 1988/04/13 00:01 [medline]
PHST- 1988/04/13 00:00 [entrez]
AID - 0014-2999(88)90114-8 [pii]
AID - 10.1016/0014-2999(88)90114-8 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1988 Apr 13;148(3):361-70. doi: 10.1016/0014-2999(88)90114-8.