PMID- 28383056
OWN - NLM
STAT- MEDLINE
DCOM- 20181116
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Apr 6
TI  - Involvement of Epithelial Na(+) Channel in the Elevated Myogenic Response in 
      Posterior Cerebral Arteries from Spontaneously Hypertensive Rats.
PG  - 45996
LID - 10.1038/srep45996 [doi]
LID - 45996
AB  - Hypertension is characterized by increased peripheral vascular resistance which 
      is related with elevated myogenic response. Recent findings have indicated that 
      epithelial sodium channel (ENaC) is involved in mechanotransduction of the 
      myogenic response. The purpose of this study was to investigate the involvement 
      of ENaC in the elevated myogenic response of posterior cerebral arteries (PCAs) 
      from spontaneously hypertensive rats (SHRs). Sixteen to eighteen weeks old male 
      wistar kyoto rats (WKYs) and SHRs were used in this study. We found that wall to 
      lumen (W/L) ratio was increased in the PCAs from SHRs compared with WKYs at the 
      resting state. Interestingly, amiloride significantly inhibited myogenic response 
      in the PCAs from SHRs and WKYs, however, the magnitude of the blockade was 
      greater in SHRs. The transfection of gammaENaC-siRNA significantly reduced the 
      expression of gammaENaC protein and inhibited myogenic response in the PCAs from 
      SHRs. Furthermore, these data were supported by the findings that 
      serum/glucocorticoid-induced kinase (Sgk1) and neural precursor cell-expressed 
      developmentally downregulated gene 4-2 (Nedd4-2) were increased in SHRs compared 
      with WKYs. Our results suggest that gammaENaC may play an important role in the 
      elevated myogenic response in PCAs from SHRs.
FAU - Choi, Soo-Kyoung
AU  - Choi SK
AD  - Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for 
      Medical Sciences, Yonsei University, Seoul, Korea.
FAU - Yeon, Soo-In
AU  - Yeon SI
AD  - Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for 
      Medical Sciences, Yonsei University, Seoul, Korea.
FAU - Kwon, Youngin
AU  - Kwon Y
AD  - Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for 
      Medical Sciences, Yonsei University, Seoul, Korea.
FAU - Byeon, Seonhee
AU  - Byeon S
AD  - Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for 
      Medical Sciences, Yonsei University, Seoul, Korea.
FAU - Lee, Young-Ho
AU  - Lee YH
AD  - Department of Physiology, College of Medicine, Brain Korea 21 Plus Project for 
      Medical Sciences, Yonsei University, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170406
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Epithelial Sodium Channels)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (RNA, Small Interfering)
RN  - 7DZO8EB0Z3 (Amiloride)
RN  - EC 2.3.2.26 (NEDD4L protein, rat)
RN  - EC 2.3.2.26 (Nedd4 Ubiquitin Protein Ligases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (serum-glucocorticoid regulated kinase)
SB  - IM
MH  - Amiloride/pharmacology
MH  - Animals
MH  - Epithelial Sodium Channels/*metabolism
MH  - Immediate-Early Proteins/metabolism
MH  - Male
MH  - *Muscle Development
MH  - Nedd4 Ubiquitin Protein Ligases/metabolism
MH  - Phosphorylation
MH  - Posterior Cerebral Artery/*metabolism
MH  - Principal Component Analysis
MH  - Protein Serine-Threonine Kinases/metabolism
MH  - Protein Subunits/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
PMC - PMC5382693
COIS- The authors declare no competing financial interests.
EDAT- 2017/04/07 06:00
MHDA- 2018/11/18 06:00
PMCR- 2017/04/06
CRDT- 2017/04/07 06:00
PHST- 2017/01/05 00:00 [received]
PHST- 2017/03/07 00:00 [accepted]
PHST- 2017/04/07 06:00 [entrez]
PHST- 2017/04/07 06:00 [pubmed]
PHST- 2018/11/18 06:00 [medline]
PHST- 2017/04/06 00:00 [pmc-release]
AID - srep45996 [pii]
AID - 10.1038/srep45996 [doi]
PST - epublish
SO  - Sci Rep. 2017 Apr 6;7:45996. doi: 10.1038/srep45996.