PMID- 28386055
OWN - NLM
STAT- MEDLINE
DCOM- 20180327
LR  - 20191113
IS  - 2325-4416 (Electronic)
IS  - 2325-4394 (Print)
IS  - 2325-4394 (Linking)
VI  - 23
DP  - 2017 Apr 7
TI  - TNF-Alpha Promotes Invasion and Metastasis via NF-Kappa B Pathway in Oral 
      Squamous Cell Carcinoma.
PG  - 141-149
AB  - BACKGROUND Recent evidence reveals that the inflammatory microenvironment is 
      associated with tumor migration, invasion, and metastasis. Tumor necrosis 
      factor-alpha (TNF-alpha) play a vital role in regulation of the inflammatory process in 
      tumor development. Nuclear factor-kappa B (NF-kappaB) is one of the key transcription 
      factors which regulate processes in tumor promotion. The aim of this study was to 
      explore the role of NF-kappaB on the invasion and migration of oral squamous cell 
      carcinoma (OSCC). MATERIAL AND METHODS The IKKbeta and p65 mRNA and protein levels 
      were determined by quantitative RT-PCR and western blot. Wound scratch healing 
      assays and transwell migration assays were used to evaluate the effect of TNF-alpha 
      and BAY11-7082 on the migration of the OSCC cell lines (HN4, HN6, and CAL27). 
      RESULTS We observed a significant increase of the expression level of IKKbeta and 
      p65 in OSCC cells from the experimental group at 24 h, 48 h, and 72 h after TNF-alpha 
      stimulation. Invasion and metastasis of OSCC cells was obviously improved after 
      the TNF-alpha stimulation. Invasion and metastasis ability of OSCC cells was 
      inhibited in the suppression group, and no significant changes were observed in 
      expression level of IKKbeta and p65 after the use of BAY11-7082. CONCLUSIONS Our 
      results suggest that TNF-alpha enhances the invasion and metastasis ability of OSCC 
      cells via the NF-kappaB signaling pathway.
FAU - Tang, Daofang
AU  - Tang D
AD  - School of Stomatology, Wannan Medical College, Wuhu, Anhui, China (mainland).
FAU - Tao, Detao
AU  - Tao D
AD  - Department of Oral and Maxillofacial Surgery, The 1st Hospital of Wannan Medical 
      College, Wuhu, Anhui, China (mainland).
FAU - Fang, Yuan
AU  - Fang Y
AD  - School of Stomatology, Wannan Medical College, Wuhu, Anhui, China (mainland).
FAU - Deng, Chao
AU  - Deng C
AD  - School of Stomatology, Wannan Medical College, Wuhu, Anhui, China (mainland).
FAU - Xu, Qing
AU  - Xu Q
AD  - School of Stomatology, Wannan Medical College, Wuhu, Anhui, China (mainland).
FAU - Zhou, Jingping
AU  - Zhou J
AD  - School of Stomatology, Wannan Medical College, Wuhu, Anhui, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20170407
PL  - United States
TA  - Med Sci Monit Basic Res
JT  - Medical science monitor basic research
JID - 101597444
RN  - 0 (I-kappa B Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
SB  - IM
MH  - Carcinoma, Squamous Cell/metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/physiology
MH  - Cell Proliferation/drug effects
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Mouth Neoplasms/*metabolism/physiopathology
MH  - NF-KappaB Inhibitor alpha/drug effects
MH  - NF-kappa B/*drug effects/*metabolism/physiology
MH  - Neoplasm Metastasis/physiopathology
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - eIF-2 Kinase/metabolism
PMC - PMC5391804
COIS- Conflict of interest None.
EDAT- 2017/04/08 06:00
MHDA- 2018/03/28 06:00
PMCR- 2017/04/07
CRDT- 2017/04/08 06:00
PHST- 2017/04/08 06:00 [entrez]
PHST- 2017/04/08 06:00 [pubmed]
PHST- 2018/03/28 06:00 [medline]
PHST- 2017/04/07 00:00 [pmc-release]
AID - 903910 [pii]
AID - 10.12659/msmbr.903910 [doi]
PST - epublish
SO  - Med Sci Monit Basic Res. 2017 Apr 7;23:141-149. doi: 10.12659/msmbr.903910.