PMID- 28386329
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 9
IP  - 3
DP  - 2017
TI  - Inhibiting endoplasmic reticulum stress by lithium chloride contributes to the 
      integrity of blood-spinal cord barrier and functional recovery after spinal cord 
      injury.
PG  - 1012-1024
AB  - Endoplasmic reticulum (ER) stress play important roles in the spinal cord injury 
      (SCI), which including blood-spinal cord barrier (BSCB) disruption. Lithium 
      chloride (LiCl) is a clinical drug for bipolar mood disorders and contributes to 
      neuroprotection. This study aims to investigate the effects of LiCl on BSCB 
      disruption and the ER stress pathway induced by spinal cord injury. We examined 
      the integrity of the BSCB with Evans Blue dye and macrophages extravasation, 
      measured the microvessels loss, the junction proteins degeneration, the 
      activation ER stress, and the locomotor function recovery. Our data indicated 
      that LiCl treatment could attenuates BSCB disruption and improved the recovery of 
      functional locomotion in rats SCI model, reduced the structure damage and number 
      loss of microvessels, increased the expressions of junction proteins, including 
      p120, beta-catenin, occludin, and claudin-5, via reversed the upregulated ER stress 
      associated proteins. In addition, LiCl significantly inhibited the increase of ER 
      stress markers and prevents loss of junction proteins in thapsigargin 
      (TG)-treated human brain microvascular endothelial cells (HBMEC). These findings 
      suggest that LiCl treatment alleviates BSCB disruption and promote the 
      neurological function recovery after SCI, partly through inhibiting the 
      activation of ER stress.
FAU - He, Zili
AU  - He Z
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical UniversityWenzhou 325035, Zhejiang, China; Molecular 
      Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      UniversityWenzhou 325035, Zhejiang, China.
FAU - Zhou, Yulong
AU  - Zhou Y
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
FAU - Wang, Qingqing
AU  - Wang Q
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical UniversityWenzhou 325035, Zhejiang, China; Molecular 
      Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      UniversityWenzhou 325035, Zhejiang, China.
FAU - Li, Jiawei
AU  - Li J
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical UniversityWenzhou 325035, Zhejiang, China; Molecular 
      Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      UniversityWenzhou 325035, Zhejiang, China.
FAU - Zheng, Zengming
AU  - Zheng Z
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
FAU - Chen, Jian
AU  - Chen J
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
FAU - Zhang, Hongyu
AU  - Zhang H
AD  - Molecular Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      University Wenzhou 325035, Zhejiang, China.
FAU - Wang, Zhouguang
AU  - Wang Z
AD  - Molecular Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      University Wenzhou 325035, Zhejiang, China.
FAU - Xu, Huazi
AU  - Xu H
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical University Wenzhou 325035, Zhejiang, China.
FAU - Xiao, Jian
AU  - Xiao J
AD  - Department of Orthopaedics, The Second Affliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical UniversityWenzhou 325035, Zhejiang, China; Molecular 
      Pharmacology Research Center, School of Pharmacy, Wenzhou Medical 
      UniversityWenzhou 325035, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20170315
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5375994
OTO - NOTNLM
OT  - Blood-spinal cord barrier
OT  - endoplasmic reticulum stress
OT  - lithium chloride
OT  - spinal cord injury
EDAT- 2017/04/08 06:00
MHDA- 2017/04/08 06:01
PMCR- 2017/03/15
CRDT- 2017/04/08 06:00
PHST- 2016/10/31 00:00 [received]
PHST- 2017/02/07 00:00 [accepted]
PHST- 2017/04/08 06:00 [entrez]
PHST- 2017/04/08 06:00 [pubmed]
PHST- 2017/04/08 06:01 [medline]
PHST- 2017/03/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2017 Mar 15;9(3):1012-1024. eCollection 2017.