PMID- 28386988
OWN - NLM
STAT- MEDLINE
DCOM- 20180222
LR  - 20231213
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 214
IP  - 4
DP  - 2017 Jun
TI  - A new proteinaceous pathogen-associated molecular pattern (PAMP) identified in 
      Ascomycete fungi induces cell death in Solanaceae.
PG  - 1657-1672
LID - 10.1111/nph.14542 [doi]
AB  - Pathogen-associated molecular patterns (PAMPs) are detected by plant pattern 
      recognition receptors (PRRs), which gives rise to PAMP-triggered immunity (PTI). 
      We characterized a novel fungal PAMP, Cell Death Inducing 1 (RcCDI1), identified 
      in the Rhynchosporium commune transcriptome sampled at an early stage of barley 
      (Hordeum vulgare) infection. The ability of RcCDI1 and its homologues from 
      different fungal species to induce cell death in Nicotiana benthamiana was tested 
      following agroinfiltration or infiltration of recombinant proteins produced by 
      Pichia pastoris. Virus-induced gene silencing (VIGS) and transient expression of 
      Phytophthora infestans effectors PiAVR3a and PexRD2 were used to assess the 
      involvement of known components of PTI in N. benthamiana responses to RcCDI1. 
      RcCDI1 was highly upregulated early during barley colonization with R. commune. 
      RcCDI1 and its homologues from different fungal species, including Zymoseptoria 
      tritici, Magnaporthe oryzae and Neurospora crassa, exhibited PAMP activity, 
      inducing cell death in Solanaceae but not in other families of dicots or 
      monocots. RcCDI1-triggered cell death was shown to require N. benthamiana 
      Brassinosteroid insensitive 1-Associated Kinase 1 (NbBAK1), N. benthamiana 
      suppressor of BIR1-1 (NbSOBIR1) and N. benthamiana SGT1 (NbSGT1), but was not 
      suppressed by PiAVR3a or PexRD2. We report the identification of a novel 
      Ascomycete PAMP, RcCDI1, recognized by Solanaceae but not by monocots, which 
      activates cell death through a pathway that is distinct from that triggered by 
      the oomycete PAMP INF1.
CI  - (c) 2017 The Authors. New Phytologist (c) 2017 New Phytologist Trust.
FAU - Franco-Orozco, Barbara
AU  - Franco-Orozco B
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
FAU - Berepiki, Adokiye
AU  - Berepiki A
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
FAU - Ruiz, Olaya
AU  - Ruiz O
AD  - Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, AL5 
      2JQ, UK.
FAU - Gamble, Louise
AU  - Gamble L
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
FAU - Griffe, Lucie L
AU  - Griffe LL
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
FAU - Wang, Shumei
AU  - Wang S
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
FAU - Birch, Paul R J
AU  - Birch PRJ
AD  - Division of Plant Sciences, University of Dundee (at JHI), Invergowrie, Dundee, 
      DD2 5DA, UK.
FAU - Kanyuka, Kostya
AU  - Kanyuka K
AUID- ORCID: 0000-0001-6324-4123
AD  - Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, AL5 
      2JQ, UK.
FAU - Avrova, Anna
AU  - Avrova A
AD  - Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee, DD2 
      5DA, UK.
LA  - eng
SI  - GENBANK/KX499545
SI  - GENBANK/KY126839
SI  - GENBANK/CZT46230.1
SI  - GENBANK/CZT04694.1
SI  - GENBANK/CZT43859.1
GR  - BB/G015244/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
DEP - 20170407
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (AVR3a protein, Phytophthora infestans)
RN  - 0 (Fungal Proteins)
RN  - 0 (Pathogen-Associated Molecular Pattern Molecules)
RN  - 0 (Plant Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Ascomycota/genetics/*pathogenicity/physiology
MH  - Cell Death
MH  - Conserved Sequence
MH  - Fungal Proteins/genetics/*metabolism
MH  - Hordeum/microbiology
MH  - Host-Pathogen Interactions/*physiology
MH  - Pathogen-Associated Molecular Pattern Molecules/*metabolism
MH  - Phylogeny
MH  - Plant Cells/microbiology
MH  - Plant Proteins/genetics/metabolism
MH  - Solanaceae/cytology/*microbiology
MH  - Nicotiana/genetics/microbiology
MH  - Virulence Factors/genetics/metabolism
OTO - NOTNLM
OT  - Rhynchosporium commune
OT  - Ascomycete
OT  - Solanaceae
OT  - cell death
OT  - elicitor
OT  - fungal
OT  - pathogen
OT  - pathogen-associated molecular pattern (PAMP)
EDAT- 2017/04/08 06:00
MHDA- 2018/02/23 06:00
CRDT- 2017/04/08 06:00
PHST- 2016/07/04 00:00 [received]
PHST- 2017/02/15 00:00 [accepted]
PHST- 2017/04/08 06:00 [pubmed]
PHST- 2018/02/23 06:00 [medline]
PHST- 2017/04/08 06:00 [entrez]
AID - 10.1111/nph.14542 [doi]
PST - ppublish
SO  - New Phytol. 2017 Jun;214(4):1657-1672. doi: 10.1111/nph.14542. Epub 2017 Apr 7.