PMID- 28390174
OWN - NLM
STAT- MEDLINE
DCOM- 20180102
LR  - 20210514
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 118
IP  - 11
DP  - 2017 Nov
TI  - Suppression of Basic Fibroblast Growth Factor Expression by Antisense 
      Oligonucleotides Inhibits Neural Stem Cell Proliferation and Differentiation in 
      Rat models With Focal Cerebral Infarction.
PG  - 3875-3882
LID - 10.1002/jcb.26038 [doi]
AB  - This study is designed to investigate the role of basic fibroblast growth factor 
      (bFGF) antisense oligonucleotide (ASODN) on the proliferation and differentiation 
      of neural stem cells (NSCs) in rat models with focal cerebral infarction (CI). 
      Seventy-five Sprague-Dawlay (SD) rats were randomly divided into the control, 
      sham, middle cerebral artery occlusion (MCAO), MCAO + nonsense oligonucleotide 
      (NODN), and MCAO + ASODN groups. Proliferation and differentiation of NSCs were 
      detected by bromodeoxyuridine (BrdU) and immunofluorescence staining, 
      respectively. ELISA was performed to detect the expressions of endogenous factors 
      that include insulin-like growth factor 1 (IGF-1), glial cell line derived 
      neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), 
      transforming growth factor-alpha1 (TGF-alpha1), bFGF, and nerve growth factor (NGF). 
      Results show significant neurological deficits and focal CI in the MCAO and 
      MCAO + NODN groups. An obvious increase of NSC proliferation, reactive 
      proliferation of astrocytes in CI areas, differentiation of newly proliferated 
      NSCs into mature neuronal cells, and expressions of endogenous growth factors 
      exhibited in the MCAO, MCAO + NODN and MCAO + ASODN groups. Compared to the MCAO 
      and MACO + NODN groups, the MCAO + ASODN group showed a significant decrease NSC 
      proliferation and differentiation in CI areas as well as decrease expressions of 
      endogenous growth factors. These findings may offer insight to help us understand 
      more as to how bFGF ASODN can effectively suppress the proliferation and 
      differentiation of NSCs. These findings are expected to help contribute to 
      research for new targets in the treatment of focal CI. J. Cell. Biochem. 118: 
      3875-3882, 2017. (c) 2017 Wiley Periodicals, Inc.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Li, Chao
AU  - Li C
AD  - Department of Neurology, The First Hospital of Jilin University, Changchun, 
      130021, P.R. China.
FAU - Che, Li-He
AU  - Che LH
AD  - Department of Infectious Diseases, The First Hospital of Jilin University, 
      Changchun, 130021,, P.R. China.
FAU - Shi, Lei
AU  - Shi L
AD  - Department of Neurosurgery, The First Hospital of Jilin University, Changchun, 
      130021,, P.R. China.
FAU - Yu, Jin-Lu
AU  - Yu JL
AD  - Department of Neurosurgery, The First Hospital of Jilin University, Changchun, 
      130021,, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20170523
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Oligodeoxyribonucleotides, Antisense)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
RIN - J Cell Biochem. 2021 Jul;122(7):770. PMID: 33988269
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Proliferation/*drug effects
MH  - Cerebral Infarction/*metabolism/pathology
MH  - Fibroblast Growth Factor 2/*antagonists & inhibitors/biosynthesis
MH  - Gene Expression Regulation/*drug effects
MH  - Neural Stem Cells/*metabolism/pathology
MH  - Oligodeoxyribonucleotides, Antisense/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - ANTISENSE OLIGONUCLEOTIDE
OT  - DIFFERENTIATION
OT  - FOCAL CEREBRAL INFARCTION
OT  - NEURAL STEM CELLS
OT  - PROLIFERATION
OT  - bFGF
EDAT- 2017/04/09 06:00
MHDA- 2018/01/03 06:00
CRDT- 2017/04/09 06:00
PHST- 2017/02/22 00:00 [received]
PHST- 2017/04/07 00:00 [accepted]
PHST- 2017/04/09 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
PHST- 2017/04/09 06:00 [entrez]
AID - 10.1002/jcb.26038 [doi]
PST - ppublish
SO  - J Cell Biochem. 2017 Nov;118(11):3875-3882. doi: 10.1002/jcb.26038. Epub 2017 May 
      23.