PMID- 28391290
OWN - NLM
STAT- MEDLINE
DCOM- 20180212
LR  - 20181113
IS  - 1941-7225 (Electronic)
IS  - 0895-7061 (Print)
IS  - 0895-7061 (Linking)
VI  - 30
IP  - 3
DP  - 2017 Mar 1
TI  - Role of Nitric Oxide Synthase on Blood Pressure Regulation and Vascular Function 
      in Pregnant Rats on a High-Fat Diet.
PG  - 240-248
LID - 10.1093/ajh/hpw153 [doi]
AB  - BACKGROUND: While obesity is a leading risk factor for preeclampsia, the 
      mechanisms whereby obese women are more susceptible to pregnancy-induced 
      hypertension are unclear. As high-fat diet (HFD) is an important contributor to 
      the development of obesity, we tested the hypothesis that pregnant rats on HFD 
      have hypertension and endothelial dysfunction due to reduced nitric oxide 
      synthase (NOS). METHODS: Twelve-week-old Sprague-Dawley female rats were fed 
      normal diet (ND, 13% fat kcal) or HFD (40% fat kcal) for 9 weeks. Timed-pregnant 
      rats were then generated and the effect of HFD on mean arterial blood pressure 
      (MAP) and vascular function was assessed on gestational day (GD) 19. RESULTS: MAP 
      was not different between HFD and ND pregnant rats. Intriguingly, sensitivity to 
      acetylcholine-induced endothelium-dependent vasorelaxation was enhanced in small 
      mesenteric arteries of HFD dams compared to ND controls (logEC50 -7.9 +/- 0.3 vs. 
      -6.7 +/- 0.3 M; P < 0.05). Additionally, HFD dams exhibited higher mesenteric 
      artery expression of NOS3 and plasma levels of NO metabolites than ND controls 
      (1738.0 +/- 316.4 vs. 1094.0 +/- 82.5 pg/mg and 72.5 +/- 8.7 vs. 39.7 +/- 4.5 microM, 
      respectively; both P < 0.05). Further, to determine the role of NOS in modulating 
      blood pressure in HFD pregnant rats, animals were treated with the nonselective 
      inhibitor Nomega-Nitro-l-arginine methyl ester hydrochloride (100 mg/l, drinking 
      water) from GD 14 to 19. It was found that NOS inhibition increased MAP equally 
      in HFD and ND groups. CONCLUSIONS: Contrary to our initial hypothesis, HFD dams 
      were normotensive and presented increased endothelial function and NO/NOS3 
      levels. This enhanced NOS-mediated vascular function does not appear to have a 
      major impact on blood pressure regulation of HFD-fed pregnant rats.
CI  - (c) American Journal of Hypertension, Ltd 2017. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com
FAU - Palei, Ana C
AU  - Palei AC
AD  - Department of Physiology and Biophysics, University of Mississippi Medical 
      Center, Jackson, Mississippi, USA.
AD  - Cardiovascular-Renal Research Center, University of Mississippi Medical Center, 
      Jackson, Mississippi, USA.
FAU - Spradley, Frank T
AU  - Spradley FT
AD  - Department of Physiology and Biophysics, University of Mississippi Medical 
      Center, Jackson, Mississippi, USA.
AD  - Cardiovascular-Renal Research Center, University of Mississippi Medical Center, 
      Jackson, Mississippi, USA.
AD  - Department of Surgery, University of Mississippi Medical Center, Jackson, 
      Mississippi, USA.
FAU - Granger, Joey P
AU  - Granger JP
AD  - Department of Physiology and Biophysics, University of Mississippi Medical 
      Center, Jackson, Mississippi, USA.
AD  - Cardiovascular-Renal Research Center, University of Mississippi Medical Center, 
      Jackson, Mississippi, USA.
LA  - eng
GR  - P01 HL051971/HL/NHLBI NIH HHS/United States
GR  - P20 GM104357/GM/NIGMS NIH HHS/United States
GR  - T32 HL105324/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Am J Hypertens
JT  - American journal of hypertension
JID - 8803676
RN  - 0 (Vasodilator Agents)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Arterial Pressure
MH  - Blood Pressure/*physiology
MH  - Blood Vessels/*physiology
MH  - Diet, High-Fat/*adverse effects
MH  - Endothelium, Vascular/physiopathology
MH  - Female
MH  - Hypertension, Pregnancy-Induced/enzymology
MH  - Mesenteric Arteries/drug effects
MH  - Nitric Oxide Synthase/*physiology
MH  - Nitric Oxide Synthase Type III/biosynthesis/genetics
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vasodilator Agents/pharmacology
PMC - PMC5861551
OTO - NOTNLM
OT  - blood pressure
OT  - endothelial function
OT  - high-fat diet
OT  - hypertension
OT  - nitric oxide
OT  - pregnancy.
EDAT- 2017/04/10 06:00
MHDA- 2018/02/13 06:00
PMCR- 2018/01/27
CRDT- 2017/04/10 06:00
PHST- 2016/06/27 00:00 [received]
PHST- 2016/11/16 00:00 [accepted]
PHST- 2017/04/10 06:00 [entrez]
PHST- 2017/04/10 06:00 [pubmed]
PHST- 2018/02/13 06:00 [medline]
PHST- 2018/01/27 00:00 [pmc-release]
AID - 2958577 [pii]
AID - hpw153 [pii]
AID - 10.1093/ajh/hpw153 [doi]
PST - ppublish
SO  - Am J Hypertens. 2017 Mar 1;30(3):240-248. doi: 10.1093/ajh/hpw153.