PMID- 28393234
OWN - NLM
STAT- MEDLINE
DCOM- 20170627
LR  - 20220317
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 37
IP  - 5
DP  - 2017 May
TI  - Downregulation of TNFAIP2 suppresses proliferation and metastasis in esophageal 
      squamous cell carcinoma through activation of the Wnt/beta-catenin signaling 
      pathway.
PG  - 2920-2928
LID - 10.3892/or.2017.5557 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays a pivotal role in malignant tumor formation 
      in the tumor microenvironment. To investigate the role of TNF-alpha in esophageal 
      squamous cell carcinoma (ESCC), we assessed expression profiles of the downstream 
      gene TNF-alpha-induced protein 2 (TNFAIP2), which e previously unknown in ESCC. 
      TNFAIP2 mRNA and protein expression levels were examined by qRT-PCR and 
      immunohistochemical analysis in 24 fresh and 55 paraffin‑embedded specimens, 
      respectively. The results demonstrated that TNFAIP2 mRNA and protein levels were 
      overexpressed in tumor cells, and TNFAIP2 overexpression was significantly 
      associated with T stage (p=0.049), N stage (p=0.019) and the International Union 
      Against Cancer (UICC) stage (p=0.028). In vitro, TNFAIP2 was highly expressed in 
      TNFalpha-stimulated Eca109, Kyse150, Kyse510 and TE-10 cells. Lentivirus-mediated RNA 
      interference of TNFAIP2 inhibited cell proliferation, colony formation, 
      migration, invasion and the cell cycle. Moreover, LV-RNAi-mediated TNFAIP2 was 
      found to regulate the Wnt/beta-catenin by decreasing expression of some genes 
      downstream from beta-catenin (i.e., C-myc, cyclin D1, MMP-7 and Snail), and 
      upregulating expression of E-cadherin and p-GSK-3beta. Taken together, these results 
      show that TNFAIP2 may be a potential tumorigenesis gene in ESCC. Our data 
      indicate that TNFAIP2 overexpression may facilitate proliferation and metastasis 
      via activation of the Wnt/beta-catenin signaling pathway in ESCC.
FAU - Xie, Yunbo
AU  - Xie Y
AD  - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, P.R. China.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170405
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Cytokines)
RN  - 0 (TNFAIP2 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cytokines/*genetics/*metabolism
MH  - Down-Regulation
MH  - Esophageal Neoplasms/genetics/*metabolism/*pathology
MH  - Esophageal Squamous Cell Carcinoma
MH  - Female
MH  - Humans
MH  - Male
MH  - Neoplasm Grading
MH  - Neoplasm Metastasis
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Wnt Signaling Pathway
EDAT- 2017/04/11 06:00
MHDA- 2017/06/28 06:00
CRDT- 2017/04/11 06:00
PHST- 2016/08/10 00:00 [received]
PHST- 2016/09/20 00:00 [accepted]
PHST- 2017/04/11 06:00 [pubmed]
PHST- 2017/06/28 06:00 [medline]
PHST- 2017/04/11 06:00 [entrez]
AID - 10.3892/or.2017.5557 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 May;37(5):2920-2928. doi: 10.3892/or.2017.5557. Epub 2017 Apr 5.