PMID- 28395594
OWN - NLM
STAT- MEDLINE
DCOM- 20171009
LR  - 20171009
IS  - 1607-8454 (Electronic)
IS  - 1024-5332 (Linking)
VI  - 22
IP  - 9
DP  - 2017 Oct
TI  - Upregulation of ADAM12 contributes to accelerated cell proliferation and cell 
      adhesion-mediated drug resistance (CAM-DR) in Non-Hodgkin's Lymphoma.
PG  - 527-535
LID - 10.1080/10245332.2017.1312205 [doi]
AB  - OBJECTIVE: ADAM12 is a member of a disintegrin and metalloproteinase family and 
      has been reported to participate in the development of variety of tumors. 
      However, the role of ADAM12 in Non-Hodgkin Lymphoma (NHL) has not been 
      investigated. The present study was undertaken to determine the expression and 
      biologic function of ADAM12 in human NHL. METHODS: First, we constructed a model 
      of cell adhesion in NHL, the mRNA, and protein level of ADAM12 in suspension and 
      the adhesion model was analyzed by RT-PCR and western blot. Then, flow cytometry 
      assay and western blot were used to investigate the mechanism of ADAM12 in the 
      proliferation of NHL cells. In vitro, after using siRNA interfering ADAM12 
      expression, we performed adhesion assay and cell viability assay to determine the 
      effect of ADAM12 on adhesive rate and drug sensitivity. RESULTS: ADAM12 was lowly 
      expressed in suspended cells and highly expressed in adherent NHL cells. In 
      addition, ADAM12 was positively correlated with the proliferation and apoptosis 
      of NHL cells by regulating the expression of p-AKT and p-GSK-3beta. Furthermore, 
      ADAM12 promoted cell adhesion-mediated drug resistance (CAM-DR) in DLBCL via AKT 
      signaling pathway. CONCLUSION AND DISCUSSION: Our data support a role for ADAM12 
      in NHL cell proliferation, adhesion, and drug resistance, and it may pave the way 
      for a novel therapeutic approach for CAM-DR in NHL.
FAU - Yin, Haibing
AU  - Yin H
AD  - a Department of Pathology , Affiliated Cancer Hospital of Nantong University , 
      Nantong , PR China.
FAU - Zhong, Fei
AU  - Zhong F
AD  - b Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Medical College, Nantong University , Nantong , PR China.
FAU - Ouyang, Yu
AU  - Ouyang Y
AD  - b Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Medical College, Nantong University , Nantong , PR China.
FAU - Wang, Qiru
AU  - Wang Q
AD  - b Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Medical College, Nantong University , Nantong , PR China.
FAU - Ding, Linlin
AU  - Ding L
AD  - b Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, 
      Medical College, Nantong University , Nantong , PR China.
FAU - He, Song
AU  - He S
AD  - a Department of Pathology , Affiliated Cancer Hospital of Nantong University , 
      Nantong , PR China.
LA  - eng
PT  - Journal Article
DEP - 20170410
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.24.- (ADAM12 Protein)
SB  - IM
MH  - ADAM12 Protein/*genetics
MH  - Animals
MH  - Antineoplastic Agents/pharmacology/therapeutic use
MH  - Apoptosis/drug effects/genetics
MH  - Biomarkers, Tumor
MH  - Cell Adhesion/*genetics
MH  - Cell Cycle/drug effects/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Drug Resistance, Neoplasm/*genetics
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glycogen Synthase Kinase 3 beta/genetics/metabolism
MH  - Humans
MH  - Lymphoma, Non-Hodgkin/drug therapy/*genetics/metabolism
MH  - Mice
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
OTO - NOTNLM
OT  - ADAM12
OT  - AKT/PI3K signaling pathway
OT  - CAM-DR
OT  - cell proliferation NHL
EDAT- 2017/04/12 06:00
MHDA- 2017/10/11 06:00
CRDT- 2017/04/12 06:00
PHST- 2017/04/12 06:00 [pubmed]
PHST- 2017/10/11 06:00 [medline]
PHST- 2017/04/12 06:00 [entrez]
AID - 10.1080/10245332.2017.1312205 [doi]
PST - ppublish
SO  - Hematology. 2017 Oct;22(9):527-535. doi: 10.1080/10245332.2017.1312205. Epub 2017 
      Apr 10.