PMID- 28397041
OWN - NLM
STAT- MEDLINE
DCOM- 20170613
LR  - 20220331
IS  - 1993-1352 (Electronic)
IS  - 1672-0733 (Linking)
VI  - 37
IP  - 2
DP  - 2017 Apr
TI  - Effects of "nourishing liver and kidney" acupuncture therapy on expression of 
      brain derived neurotrophic factor and synaptophysin after cerebral ischemia 
      reperfusion in rats.
PG  - 271-278
LID - 10.1007/s11596-017-1727-7 [doi]
AB  - The aim of the present study was to investigate the effect of "nourishing liver 
      and kidney" acupuncture therapy on motor and cognitive deficits, and the 
      underlying mechanism following cerebral ischemia-reperfusion (I/R) via increasing 
      the expression of brain derived neurotrophic factor (BDNF) and synaptophysin 
      (SYN) in the hippocampus. Healthy adult male SD rats were randomly divided into 
      sham operation group (n=51), model group (n=51), acupuncture group (n=51) and 
      acupuncture control group (n=51). The middle cerebral I/R model was established. 
      Acupunctures were performed in the acupuncture group and acupuncture control 
      group at acupoints of Taixi (K103), Taichong (ST09) of both sides, for 30 min 
      once daily every morning. The animals in the sham operation group and model group 
      were conventionally fed in the cage, without any intervention therapy. The rats 
      of each group were assessed with modified neurological severity scores (mNSS). 
      The expression of BDNF and SYN in the hippocampus was detected by 
      immunohistochemical SP method and the synaptic structure in hippocampus area was 
      assessed morphologically and quantitatively at the 3rd, 7th and 14th day. The 
      Morris water Maze (MWM) test was used to evaluate the rats' learning and memory 
      abilities on the 15th day after acupuncture. The animals in the acupuncture 
      control group and sham operation group presented no neurological deficit. In the 
      acupuncture group, the nerve functional recovery was significantly better than 
      that in the model group at the 7th and 14th day after modeling. The average MWM 
      escape latency in the acupuncture group was shorter than that in the model group 
      at the 3rd, 4th and 5th day. The number of crossings of the platform quadrant in 
      the acupuncture group was significantly more than that in the model group. At the 
      each time point, the expression levels of BDNF and SYN in the hippocampal regions 
      increased significantly in the model group as compared with the sham operation 
      group and the acupuncture control group. In the acupuncture group, the expression 
      levels of BDNF at the 7th and 14th day increased more significantly than those in 
      the model group. In the acupuncture group, the expression levels of SYN at the 
      each time point increased more significantly than those in the model group. The 
      post-synaptic density (PSD) was significantly increased and the synapse cleft 
      width was narrowed in the acupuncture group as compared with other groups. The 
      synaptic curvatures were improved obviously in the acupuncture group in contrast 
      to the model group. It was concluded that the "nourishing liver and kidney" 
      acupuncture therapy has positive effects on behavioral recovery, as well as 
      learning and memory abilities, probably by promoting the expression of BDNF and 
      SYN, and synaptic structure reconstruction in the ipsilateral hippocampus after 
      I/R in rats. The "nourishing liver and kidney" acupuncture therapy can promote 
      the functional recovery in rats after cerebral ischemia injury.
FAU - Xia, Wen-Guang
AU  - Xia WG
AD  - Department of Rehabilitation Medicine, Hubei Provincial Hospital of the 
      Integrated Chinese and Western Medicine, Wuhan, 430015, China. docxwg@163.com.
FAU - Zheng, Chan-Juan
AU  - Zheng CJ
AD  - Department of Rehabilitation Medicine, Hubei Provincial Hospital of the 
      Integrated Chinese and Western Medicine, Wuhan, 430015, China.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - Department of Rehabilitation Medicine, Hubei Provincial Hospital of the 
      Integrated Chinese and Western Medicine, Wuhan, 430015, China.
FAU - Wang, Juan
AU  - Wang J
AD  - Department of Rehabilitation Medicine, Hubei Provincial Hospital of the 
      Integrated Chinese and Western Medicine, Wuhan, 430015, China.
LA  - eng
PT  - Journal Article
DEP - 20170411
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Synaptophysin)
SB  - IM
MH  - Acupuncture Points
MH  - Acupuncture Therapy
MH  - Animals
MH  - Brain Ischemia/metabolism/psychology/*therapy
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Disease Models, Animal
MH  - Hippocampus/metabolism/*pathology
MH  - Male
MH  - Maze Learning
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/metabolism/psychology/*therapy
MH  - Synaptophysin/*metabolism
OTO - NOTNLM
OT  - acupuncture
OT  - brain derived neurotrophic factor
OT  - cerebral ischemia reperfusion
OT  - synaptophysin
EDAT- 2017/04/12 06:00
MHDA- 2017/06/14 06:00
CRDT- 2017/04/12 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/03/06 00:00 [revised]
PHST- 2017/04/12 06:00 [entrez]
PHST- 2017/04/12 06:00 [pubmed]
PHST- 2017/06/14 06:00 [medline]
AID - 10.1007/s11596-017-1727-7 [pii]
AID - 10.1007/s11596-017-1727-7 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2017 Apr;37(2):271-278. doi: 
      10.1007/s11596-017-1727-7. Epub 2017 Apr 11.