PMID- 2839715
OWN - NLM
STAT- MEDLINE
DCOM- 19880819
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 62
IP  - 8
DP  - 1988 Aug
TI  - Alterations in the U3 region of the long terminal repeat of an infectious 
      thymotropic type B retrovirus.
PG  - 2985-93
AB  - We isolated and characterized a type B thymotropic retrovirus (DMBA-LV) which is 
      highly related to mouse mammary tumor virus (MMTV) isolates and which induces 
      T-cell thymomas with a high incidence and a very short latent period. Regions of 
      nonhomology between the DMBA-LV genome and the MMTV genome were identified by 
      heteroduplex mapping and nucleotide sequence studies. In the electron microscope 
      heteroduplex mapping studies the EcoRI-generated 5' and 3' fragments of the 
      DMBA-LV genome were compared with the corresponding fragments of the MMTV (C3H 
      and GR) genome isolated from mammary tumors. The results indicated that DMBA-LV 
      contained a region of nonhomologous nucleotide sequences in the 3' half of the U3 
      region of the long terminal repeat (LTR). Nucleotide sequence studies confirmed 
      these results and showed that in this region 440 nucleotides of the MMTV (C3H) 
      sequences were deleted and substituted with a segment of 122 nucleotides. This 
      substituted segment in the form of a tandem repeat structure contained nucleotide 
      sequences derived exclusively from sequences which flanked the substitution loop. 
      The distal glucocorticoid regulatory element was unaltered, and two additional 
      copies of the distal glucocorticoid regulatory element-binding site were present 
      in the substituted region. The restriction endonuclease map of the reconstructed 
      molecular clone of DMBA-LV was identical to that corresponding to unintegrated 
      linear DMBA-LV DNA present in DMBA-LV-induced tumor cell lines. Since the 
      nucleotide sequences of the LTRs present in four different DMBA-LV proviral 
      copies isolated from a single thymoma were identical, we concluded that they were 
      derived from the same parental virus and that this type B retrovirus containing 
      an alteration in the U3 region of its LTR could induce thymic lymphomas. Thus, 
      DMBA-LV represents the first example of a productively replicating type B 
      retrovirus that contains an LTR modified in the U3 region and that has target 
      cell and disease specificity for T cells.
FAU - Ball, J K
AU  - Ball JK
AD  - Department of Biochemistry, University of Western Ontario, London, Canada.
FAU - Diggelmann, H
AU  - Diggelmann H
FAU - Dekaban, G A
AU  - Dekaban GA
FAU - Grossi, G F
AU  - Grossi GF
FAU - Semmler, R
AU  - Semmler R
FAU - Waight, P A
AU  - Waight PA
FAU - Fletcher, R F
AU  - Fletcher RF
LA  - eng
SI  - GENBANK/M20790
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - DNA Restriction Enzymes
MH  - DNA, Viral/genetics
MH  - Genes, Viral
MH  - Lymphoma/*microbiology
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - Regulatory Sequences, Nucleic Acid
MH  - Repetitive Sequences, Nucleic Acid
MH  - Retroviridae/*genetics/pathogenicity
MH  - Sequence Homology, Nucleic Acid
MH  - T-Lymphocytes/*microbiology
MH  - Virus Replication
PMC - PMC253737
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
PMCR- 1988/08/01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
PHST- 1988/08/01 00:00 [pmc-release]
AID - 10.1128/JVI.62.8.2985-2993.1988 [doi]
PST - ppublish
SO  - J Virol. 1988 Aug;62(8):2985-93. doi: 10.1128/JVI.62.8.2985-2993.1988.