PMID- 28397501
OWN - NLM
STAT- MEDLINE
DCOM- 20190109
LR  - 20240327
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Print)
IS  - 1944-8244 (Linking)
VI  - 9
IP  - 17
DP  - 2017 May 3
TI  - Layer-by-Layer-Assembled Laccase Enzyme on Stimuli-Responsive Membranes for 
      Chloro-Organics Degradation.
PG  - 14858-14867
LID - 10.1021/acsami.7b01999 [doi]
AB  - Functionalized membranes provide versatile platforms for the incorporation of 
      biocatalysts and nanostructured materials for efficient and benign environmental 
      remediation. The existing techniques for remediating chloro-organics in water 
      consist of both physical and chemical means mostly using metal oxide-based 
      catalysts, despite associated environmental concerns. To offer bioinspired 
      remediation as an alternative, we herein demonstrate a layer-by-layer approach to 
      immobilize laccase enzyme onto pH-responsive functionalized membranes for the 
      degradation of chloro-organics in water. The efficacy of these bioinspired 
      membranes toward dechlorination of 2,4,6-trichlorophenol (TCP) is demonstrated 
      under a pressure-driven continuous flow mode (convective flow) for the first time 
      to the best of our knowledge. Over 80% of the initial TCP was degraded at an 
      optimum flow rate under an applied air pressure of about 0.7 bar or lower. This 
      corresponds to degradation of a substantial amount of the initial substrate in 
      only 36 s residence time, whereas it takes hours for degradation in a batch 
      reaction. This, in fact, demonstrates an energy efficient flow-through system 
      with potentially large-scale applications. Comparison of the stability of the 
      enzyme in the solution phase versus immobilized on the membrane phase showed a 
      loss of some 65% of enzyme activity in the solution phase after 22 d, whereas the 
      membrane-bound enzyme lost only a negligible percentage of the activity in a 
      comparable time span. Finally, the membrane was exposed to rigorous cycles of TCP 
      degradation trials to study its reusability. The primary results reveal a loss of 
      only 14% of the initial activity after 4 cycles of use in a period of 25 d, 
      demonstrating its potential to be reused. Regeneration of the functionalized 
      membrane was also validated by dislodging the immobilized enzyme, followed by 
      immobilization of fresh enzyme onto the membrane.
FAU - Sarma, Rupam
AU  - Sarma R
AUID- ORCID: 0000-0003-3539-5949
AD  - Department of Chemical and Materials Engineering, University of Kentucky , 177 F. 
      Paul Anderson Tower, Lexington, Kentucky 40506, United States.
FAU - Islam, Md Saiful
AU  - Islam MS
AD  - Department of Chemical and Materials Engineering, University of Kentucky , 177 F. 
      Paul Anderson Tower, Lexington, Kentucky 40506, United States.
FAU - Miller, Anne-Frances
AU  - Miller AF
AD  - Department of Chemical and Materials Engineering, University of Kentucky , 177 F. 
      Paul Anderson Tower, Lexington, Kentucky 40506, United States.
FAU - Bhattacharyya, Dibakar
AU  - Bhattacharyya D
AUID- ORCID: 0000-0001-9948-9085
AD  - Department of Chemical and Materials Engineering, University of Kentucky , 177 F. 
      Paul Anderson Tower, Lexington, Kentucky 40506, United States.
LA  - eng
GR  - P42 ES007380/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20170421
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Chlorophenols
MH  - Enzyme Stability
MH  - Enzymes, Immobilized
MH  - Laccase/*metabolism
MH  - Membranes
PMC - PMC5787852
MID - NIHMS936573
OTO - NOTNLM
OT  - catalysis
OT  - enzyme immobilization
OT  - functionalized membrane
OT  - laccase
OT  - layer-by-layer assembly
OT  - trichlorophenol
EDAT- 2017/04/12 06:00
MHDA- 2019/01/10 06:00
PMCR- 2018/05/03
CRDT- 2017/04/12 06:00
PHST- 2017/04/12 06:00 [pubmed]
PHST- 2019/01/10 06:00 [medline]
PHST- 2017/04/12 06:00 [entrez]
PHST- 2018/05/03 00:00 [pmc-release]
AID - 10.1021/acsami.7b01999 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2017 May 3;9(17):14858-14867. doi: 
      10.1021/acsami.7b01999. Epub 2017 Apr 21.