PMID- 28398169
OWN - NLM
STAT- MEDLINE
DCOM- 20180309
LR  - 20180508
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 26
IP  - 12
DP  - 2017 Jun 15
TI  - Paving the Way Toward Complex Blood-Brain Barrier Models Using Pluripotent Stem 
      Cells.
PG  - 857-874
LID - 10.1089/scd.2017.0003 [doi]
AB  - A tissue with great need to be modeled in vitro is the blood-brain barrier (BBB). 
      The BBB is a tight barrier that covers all blood vessels in the brain and 
      separates the brain microenvironment from the blood system. It consists of three 
      cell types [neurovascular unit (NVU)] that contribute to the unique tightness and 
      selective permeability of the BBB and has been shown to be disrupted in many 
      diseases and brain disorders, such as vascular dementia, stroke, multiple 
      sclerosis, and Alzheimer's disease. Given the progress that pluripotent stem 
      cells (PSCs) have made in the past two decades, it is now possible to produce 
      many cell types from the BBB and even partially recapitulate this complex tissue 
      in vitro. In this review, we summarize the most recent developments in PSC 
      differentiation and modeling of the BBB. We also suggest how patient-specific 
      human-induced PSCs could be used to model BBB dysfunction in the future. Lastly, 
      we provide perspectives on how to improve production of the BBB in vitro, for 
      example by improving pericyte differentiation protocols and by better modeling 
      the NVU in the dish.
FAU - Lauschke, Karin
AU  - Lauschke K
AD  - 1 National Food Institute, Technical University of Denmark , Kongens Lyngby, 
      Denmark .
AD  - 2 Department of Micro- and Nanotechnology, Technical University of Denmark , 
      Kongens Lyngby, Denmark .
FAU - Frederiksen, Lise
AU  - Frederiksen L
AD  - 3 Faculty of Health and Medical Sciences, University of Copenhagen , Kobenhavn N, 
      Denmark .
FAU - Hall, Vanessa Jane
AU  - Hall VJ
AD  - 4 Department of Veterinary and Animal Sciences, Faculty of Health and Medical 
      Sciences, University of Copenhagen , Frederiksberg C, Denmark .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20170517
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
SB  - IM
MH  - Alzheimer Disease/*metabolism/pathology
MH  - Animals
MH  - Blood-Brain Barrier/*metabolism/pathology
MH  - Dementia, Vascular/*metabolism/pathology
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*metabolism/pathology
MH  - *Models, Cardiovascular
MH  - Multiple Sclerosis/*metabolism/pathology
OTO - NOTNLM
OT  - bioprinting
OT  - blood-brain barrier
OT  - in vitro model
OT  - induced pluripotent stem cell
OT  - neurodegenerative disease
OT  - scaffold
EDAT- 2017/04/12 06:00
MHDA- 2018/03/10 06:00
CRDT- 2017/04/12 06:00
PHST- 2017/04/12 06:00 [pubmed]
PHST- 2018/03/10 06:00 [medline]
PHST- 2017/04/12 06:00 [entrez]
AID - 10.1089/scd.2017.0003 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2017 Jun 15;26(12):857-874. doi: 10.1089/scd.2017.0003. Epub 2017 
      May 17.