PMID- 28400267
OWN - NLM
STAT- MEDLINE
DCOM- 20170608
LR  - 20170608
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 618
DP  - 2017 Jun 30
TI  - CHD5 is a potential tumor suppressor in non small cell lung cancer (NSCLC).
PG  - 65-68
LID - S0378-1119(17)30253-6 [pii]
LID - 10.1016/j.gene.2017.04.010 [doi]
AB  - Lung cancer is one of the deadliest types of cancers and genetic and epigenetic 
      alterations play major roles in its development. Chromodomain (CHD) protein 
      family acts in chromatin organization, regulation of transcription and also 
      genomic stability and cancer prevention. Although CHD5, a member of this family 
      was shown to contribute to major cellular events and functions as a tumor 
      suppressor gene in various types of cancer, it is not clear whether CHD5 plays a 
      role in lung carcinogenesis. The aim of this study was to investigate the 
      possible role of CHD5 in progression of non-small cell lung cancer (NSCLC). 
      Expression levels of CHD5 gene in 59 tumor and corresponding non-cancerous lung 
      tissue samples were analyzed by qRT-PCR and the methylation status of the 
      promoter region was investigated by methylation specific PCR (MS-PCR). The Akt 
      phosphorylation levels were investigated by Western Blot (WB). CHD5 was 
      down-regulated in 17 (39.5%) and up-regulated in 24 (55.8%) of tumor specimens. 
      Even though the promoter of CHD5 was hypermethylated in 8 patients, it was not 
      found associated with CHD5 gene expression (p=0.08). Akt phosphorylation was 
      increased in 14 (53.8%) and decreased in 12 (46.2%) of the samples but no 
      significant association was found between p-Akt phosphorylation and CHD5 
      expression (p=0.67). We suggest that CHD5 may act as a tumor suppressor gene in 
      NSCLC.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Baykara, Onur
AU  - Baykara O
AD  - Istanbul University Cerrahpasa Medical Faculty, Dept. of Medical Biology and 
      Genetics, Istanbul 34098, Turkey.
FAU - Tansarikaya, Merve
AU  - Tansarikaya M
AD  - Istanbul University Cerrahpasa Medical Faculty, Dept. of Medical Biology and 
      Genetics, Istanbul 34098, Turkey.
FAU - Bulut, Pelin
AU  - Bulut P
AD  - Istanbul University Cerrahpasa Medical Faculty, Dept. of Medical Biology and 
      Genetics, Istanbul 34098, Turkey.
FAU - Demirkaya, Ahmet
AU  - Demirkaya A
AD  - Istanbul University Cerrahpasa Medical Faculty, Dept. of Thoracic Surgery, 
      Istanbul 34098, Turkey.
FAU - Buyru, Nur
AU  - Buyru N
AD  - Istanbul University Cerrahpasa Medical Faculty, Dept. of Medical Biology and 
      Genetics, Istanbul 34098, Turkey. Electronic address: nsayhan@istanbul.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20170408
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (CHD5 protein, human)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/metabolism/pathology
MH  - DNA Helicases/*genetics/metabolism
MH  - DNA Methylation
MH  - Female
MH  - Humans
MH  - Lung Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Promoter Regions, Genetic
OTO - NOTNLM
OT  - CHD5
OT  - Chromodomain
OT  - Lung cancer
OT  - NSCLC
EDAT- 2017/04/13 06:00
MHDA- 2017/06/09 06:00
CRDT- 2017/04/13 06:00
PHST- 2016/12/21 00:00 [received]
PHST- 2017/03/17 00:00 [revised]
PHST- 2017/04/07 00:00 [accepted]
PHST- 2017/04/13 06:00 [pubmed]
PHST- 2017/06/09 06:00 [medline]
PHST- 2017/04/13 06:00 [entrez]
AID - S0378-1119(17)30253-6 [pii]
AID - 10.1016/j.gene.2017.04.010 [doi]
PST - ppublish
SO  - Gene. 2017 Jun 30;618:65-68. doi: 10.1016/j.gene.2017.04.010. Epub 2017 Apr 8.