PMID- 28402841
OWN - NLM
STAT- MEDLINE
DCOM- 20171226
LR  - 20171226
IS  - 1873-426X (Electronic)
IS  - 0008-6215 (Linking)
VI  - 443-444
DP  - 2017 Apr 18
TI  - Identification and biochemical characterization of a novel 
      alpha-1,3-mannosyltransferase WfcD from Escherichia coli O141.
PG  - 78-86
LID - S0008-6215(17)30019-8 [pii]
LID - 10.1016/j.carres.2017.04.003 [doi]
AB  - Glycosyltransferases (GTs) catalyze the formation of regio- and stereospecific 
      glycosidic linkages between specific sugar donors and recipients. In this study, 
      the function of the wfcD gene from the Escherichia coli O141 O-antigen gene 
      cluster encoding an alpha-1,3-mannosyltransferase that catalyzed the formation of the 
      linkage Man(alpha1-3)-GlcNAc was biochemically characterized. WfcD was expressed in 
      E. coli BL21 (DE3), and the enzymatic product was identified by liquid 
      chromatography-mass spectrometry (LC-MS), collision-induced dissociation 
      electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MS(n)) and 
      glycosidase digestion using the donor substrate GDP-Man and the synthetic 
      acceptor substrate decyl diphosphate 2-acetamido-2-deoxy-alpha-D-glucopyranose 
      (GlcNAc-PP-De). The kinetic and physiochemical properties and the substrate 
      specificity of WfcD were investigated. WfcD is the first characterized bacterial 
      mannosyltransferase that acts on the Man(alpha1-3)-GlcNAc linkage. This study 
      enhances our knowledge of the diverse functions of GTs.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Chen, Chao
AU  - Chen C
AD  - TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 
      Hongda Street, TEDA, Tianjin, 300457, PR China; The Key Laboratory of Molecular 
      Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, 
      Tianjin, 300457, PR China.
FAU - Hou, Xi
AU  - Hou X
AD  - TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 
      Hongda Street, TEDA, Tianjin, 300457, PR China; The Key Laboratory of Molecular 
      Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, 
      Tianjin, 300457, PR China.
FAU - Utkina, Natalia
AU  - Utkina N
AD  - N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 
      Moscow, Russian Federation.
FAU - Danilov, Leonid
AU  - Danilov L
AD  - N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 
      Moscow, Russian Federation.
FAU - Zhou, Dawei
AU  - Zhou D
AD  - TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 
      Hongda Street, TEDA, Tianjin, 300457, PR China; The Key Laboratory of Molecular 
      Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, 
      Tianjin, 300457, PR China.
FAU - Torgov, Vladimir
AU  - Torgov V
AD  - N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 
      Moscow, Russian Federation.
FAU - Veselovsky, Vladimir
AU  - Veselovsky V
AD  - N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 
      Moscow, Russian Federation.
FAU - Liu, Bin
AU  - Liu B
AD  - TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 
      Hongda Street, TEDA, Tianjin, 300457, PR China; The Key Laboratory of Molecular 
      Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, 
      Tianjin, 300457, PR China.
FAU - Feng, Lu
AU  - Feng L
AD  - TEDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 
      Hongda Street, TEDA, Tianjin, 300457, PR China; The Key Laboratory of Molecular 
      Microbiology and Technology, Ministry of Education, 23 Hongda Street, TEDA, 
      Tianjin, 300457, PR China. Electronic address: fenglu63@nankai.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170406
PL  - Netherlands
TA  - Carbohydr Res
JT  - Carbohydrate research
JID - 0043535
RN  - 0 (Detergents)
RN  - 0 (Metals)
RN  - 0 (O Antigens)
RN  - EC 2.4.1.- (Mannosyltransferases)
SB  - IM
MH  - Biocatalysis
MH  - Carbohydrate Sequence
MH  - Detergents/pharmacology
MH  - Escherichia coli/*enzymology
MH  - Kinetics
MH  - Mannosyltransferases/*metabolism
MH  - Metals/pharmacology
MH  - O Antigens/chemistry/metabolism
MH  - Substrate Specificity
OTO - NOTNLM
OT  - Escherichia coli
OT  - Mannosyltransferase
OT  - Mass spectrometry
OT  - WfcD
EDAT- 2017/04/14 06:00
MHDA- 2017/12/27 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/01/12 00:00 [received]
PHST- 2017/03/31 00:00 [revised]
PHST- 2017/04/02 00:00 [accepted]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/12/27 06:00 [medline]
PHST- 2017/04/14 06:00 [entrez]
AID - S0008-6215(17)30019-8 [pii]
AID - 10.1016/j.carres.2017.04.003 [doi]
PST - ppublish
SO  - Carbohydr Res. 2017 Apr 18;443-444:78-86. doi: 10.1016/j.carres.2017.04.003. Epub 
      2017 Apr 6.