PMID- 28404953
OWN - NLM
STAT- MEDLINE
DCOM- 20180406
LR  - 20201209
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 26
DP  - 2017 Jun 27
TI  - Hydrogen-rich saline attenuates spinal cord hemisection-induced testicular injury 
      in rats.
PG  - 42314-42331
LID - 10.18632/oncotarget.15876 [doi]
AB  - To study how hydrogen-rich saline (HS) promotes the recovery of testicular 
      biological function in a hemi-sectioned spinal cord injury (hSCI) rat model, a 
      right hemisection was performed at the T11-T12 of the spinal cord in Wistar rats. 
      Animals were divided into four groups: normal group; vehicle group: sham-operated 
      rats administered saline; hSCI group: subjected to hSCI and administered saline; 
      HRST group: subjected to hSCI and administered HS. Hind limb neurological 
      function, testis index, testicular morphology, mean seminiferous tubular diameter 
      (MSTD) and seminiferous epithelial thickness (MSET), the expression of heme 
      oxygenase-1 (HO-1), mitofusin-2 (MFN-2), and high-mobility group box 1 (HMGB-1), 
      cell ultrastructure, and apoptosis of spermatogenic cells were studied. The 
      results indicated that hSCI significantly decreased the hind limb neurological 
      function, testis index, MSTD, and MSET, and induced severe testicular 
      morphological injury. The MFN-2 level was decreased, and HO-1 and HMGB-1 were 
      overexpressed in testicular tissues. In addition, hSCI accelerated the apoptosis 
      of spermatogenic cells and the ultrastructural damage of cells in the hypophysis 
      and testis. After HS administration, all these parameters were considerably 
      improved, and the characteristics of hSCI testes were similar to those of normal 
      control testes. Taken together, HS administration can promote the recovery of 
      testicular biological function by anti-oxidative, anti-inflammatory, and 
      anti-apoptotic action. More importantly, HS can inhibit the hSCI-induced 
      ultrastructural changes in gonadotrophs, ameliorate the abnormal regulation of 
      the hypothalamic-pituitary-testis axis, and thereby promote the recovery of 
      testicular injury. HS administration also inhibited the hSCI-induced 
      ultrastructural changes in testicular spermatogenic cells, Sertoli cells and 
      interstitial cells.
FAU - Ge, Li
AU  - Ge L
AD  - Department of Histology and Embryology, Taishan Medical University, Tai-an City, 
      PR China.
FAU - Wei, Li-Hua
AU  - Wei LH
AD  - Department of Histology and Embryology, Taishan Medical University, Tai-an City, 
      PR China.
FAU - Du, Chang-Qing
AU  - Du CQ
AD  - Department of Histology and Embryology, Taishan Medical University, Tai-an City, 
      PR China.
FAU - Song, Guo-Hua
AU  - Song GH
AD  - Key Laboratory of Atherosclerosis in Universities of Shandong, Taishan Medical 
      University, Institute of Atherosclerosis, Taishan Medical University, Tai-an 
      City, PR China.
FAU - Xue, Ya-Zhuo
AU  - Xue YZ
AD  - Department of Basic Nursing Teaching, Taishan Medical University, Tai-an City, PR 
      China.
FAU - Shi, Hao-Shen
AU  - Shi HS
AD  - Department of Clinical Medicine, Taishan Medical University, Tai-an City, PR 
      China.
FAU - Yang, Ming
AU  - Yang M
AD  - Department of Clinical Medicine, Taishan Medical University, Tai-an City, PR 
      China.
FAU - Yin, Xin-Xin
AU  - Yin XX
AD  - Department of Clinical Medicine, Taishan Medical University, Tai-an City, PR 
      China.
FAU - Li, Run-Ting
AU  - Li RT
AD  - Department of Clinical Medicine, Taishan Medical University, Tai-an City, PR 
      China.
FAU - Wang, Xue-Er
AU  - Wang XE
AD  - Department of Physiology, Shandong University School of Medicine, Jinan, 
      Shandong, PR China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Physiology, Shandong University School of Medicine, Jinan, 
      Shandong, PR China.
FAU - Song, Wen-Gang
AU  - Song WG
AD  - Department of Medical Immunology, Taishan Medical University, Tai-an City, PR 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
RN  - 0 (Biomarkers)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (Mfn2 protein, rat)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Biomarkers
MH  - Disease Models, Animal
MH  - GTP Phosphohydrolases
MH  - Gene Expression
MH  - Germ Cells/drug effects/metabolism
MH  - Heme Oxygenase-1/genetics/metabolism
MH  - Hydrogen/*administration & dosage
MH  - Male
MH  - Membrane Proteins/genetics/metabolism
MH  - Mitochondrial Proteins/genetics/metabolism
MH  - Neurological Rehabilitation
MH  - Pituitary Gland/drug effects/ultrastructure
MH  - Rats
MH  - Recovery of Function/drug effects
MH  - *Saline Waters
MH  - Sertoli Cells/drug effects/metabolism/ultrastructure
MH  - Spinal Cord Injuries/*complications/diagnosis
MH  - Testicular Diseases/drug therapy/*etiology/metabolism/*rehabilitation
MH  - Testis/drug effects/metabolism/physiopathology/ultrastructure
PMC - PMC5522069
OTO - NOTNLM
OT  - hemisectioned spinal cord injury
OT  - hydrogen-rich saline
OT  - rat
OT  - spermatogenic cell
OT  - testis
COIS- CONFLICTS OF INTEREST The authors declare no competing interest.
EDAT- 2017/04/14 06:00
MHDA- 2018/04/07 06:00
PMCR- 2017/06/27
CRDT- 2017/04/14 06:00
PHST- 2016/07/20 00:00 [received]
PHST- 2017/01/27 00:00 [accepted]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2018/04/07 06:00 [medline]
PHST- 2017/04/14 06:00 [entrez]
PHST- 2017/06/27 00:00 [pmc-release]
AID - 15876 [pii]
AID - 10.18632/oncotarget.15876 [doi]
PST - ppublish
SO  - Oncotarget. 2017 Jun 27;8(26):42314-42331. doi: 10.18632/oncotarget.15876.