PMID- 28405131
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230108
IS  - 0976-500X (Print)
IS  - 0976-5018 (Electronic)
IS  - 0976-500X (Linking)
VI  - 8
IP  - 1
DP  - 2017 Jan-Mar
TI  - Indocyanine Green-001 (ICG-001) Attenuates Wnt/beta-catenin-induces Myocardial 
      Injury Following Sepsis.
PG  - 14-20
LID - 10.4103/jpp.JPP_153_16 [doi]
AB  - OBJECTIVE: To investigate the mechanistic pathway of both indocyanine green 
      (ICG)-001 in attenuated endotoxemia-induced cardiac depression through 
      downregulation cardiac Wnt/ beta-catenin cell signaling. MATERIALS AND METHODS: 
      Adult (4-6 months) male Albino-Webster mice, their weights ranged from 25 to 30 
      g, were pretreated with ICG-001 i.p., following cecal ligation and puncture 
      (CLP). Left ventricle (LV) function was assessed using a microcatheter system. 
      Monocyte chemoattractant protein-1 (MCP-1) and cytokines mediators in plasma and 
      myocardium were analyzed by enzyme-linked immunosorbent assay. Further, the 
      cardiac Wnt protein measured by quantitative real-time polymerase chain reaction 
      while beta-catenin analysis through Western blotting procedure. The pathological 
      changes and cells injury in the myocardium were examined using hematoxylin and 
      eosin staining. RESULTS: CLP mice displayed worse LV function. The exaggerated 
      cardiac depression in CLP mice was associated with higher levels of MCP-1 and 
      cytokines in plasma and myocardium together with greater cardiac levels of 
      cardiac troponin-I and Wnt/beta-catenin. Neutralization of sepsis by either 
      ICG-001resulted in improved LV function and reductions in inflammatory mediators. 
      CONCLUSION: Taken together, these data showed that ICG-001 improved LV function 
      following sepsis through downregulation of Wnt/beta-catenin and serve as a potential 
      mechanistic pathway ICG-001 in therapeutic cardiac endotoxemia in animal model.
FAU - Yousif, Nasser Ghaly
AU  - Yousif NG
AD  - Department of Pharmacology and Therapeutics, College of Medicine, University of 
      Kufa, Kufa, Iraq.
FAU - Hadi, Najah R
AU  - Hadi NR
AD  - Department of Pharmacology and Therapeutics, College of Medicine, University of 
      Kufa, Kufa, Iraq.
FAU - Hassan, Alaa Manea
AU  - Hassan AM
AD  - Department of Pharmacology and Therapeutics, College of Medicine, University of 
      Kufa, Kufa, Iraq.
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Pharmacol Pharmacother
JT  - Journal of pharmacology & pharmacotherapeutics
JID - 101552113
PMC - PMC5370323
OTO - NOTNLM
OT  - Indocyanine green-100
OT  - myocardial injury
OT  - sepsis
COIS- There are no conflicts of interest.
EDAT- 2017/04/14 06:00
MHDA- 2017/04/14 06:01
PMCR- 2017/01/01
CRDT- 2017/04/14 06:00
PHST- 2017/04/14 06:00 [entrez]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/04/14 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - JPP-8-14 [pii]
AID - 10.4103/jpp.JPP_153_16 [doi]
PST - ppublish
SO  - J Pharmacol Pharmacother. 2017 Jan-Mar;8(1):14-20. doi: 10.4103/jpp.JPP_153_16.