PMID- 28405258
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1932-1058 (Print)
IS  - 1932-1058 (Electronic)
IS  - 1932-1058 (Linking)
VI  - 11
IP  - 2
DP  - 2017 Mar
TI  - Rapid isolation of blood plasma using a cascaded inertial microfluidic device.
PG  - 024109
LID - 10.1063/1.4979198 [doi]
LID - 024109
AB  - Blood, saliva, mucus, sweat, sputum, and other biological fluids are often 
      hindered in their ability to be used in point-of-care (POC) diagnostics because 
      their assays require some form of off-site sample pre-preparation to effectively 
      separate biomarkers from larger components such as cells. The rapid isolation, 
      identification, and quantification of proteins and other small molecules 
      circulating in the blood plasma from larger interfering molecules are therefore 
      particularly important factors for optical blood diagnostic tests, in particular, 
      when using optical approaches that incur spectroscopic interference from 
      hemoglobin-rich red blood cells (RBCs). In this work, a sequential spiral 
      polydimethylsiloxane (PDMS) microfluidic device for rapid ( approximately 1 min) on-chip blood 
      cell separation is presented. The chip utilizes Dean-force induced migration via 
      two 5-loop Archimedean spirals in series. The chip was characterized in its 
      ability to filter solutions containing fluorescent beads and silver nanoparticles 
      and further using blood solutions doped with a fluorescent protein. Through these 
      experiments, both cellular and small molecule behaviors in the chip were 
      assessed. The results exhibit an average RBC separation efficiency of  approximately 99% at a 
      rate of 5.2 x 10(6) cells per second while retaining 95% of plasma components. 
      This chip is uniquely suited for integration within a larger point-of-care 
      diagnostic system for the testing of blood plasma, and the use of multiple 
      filtering spirals allows for the tuning of filtering steps, making this device 
      and the underlying technique applicable for a wide range of separation 
      applications.
FAU - Robinson, M
AU  - Robinson M
AD  - Department of Biomedical Engineering, Texas A&M University , College Station, 
      Texas 77843, USA.
FAU - Marks, H
AU  - Marks H
AUID- ORCID: 0000-0001-7407-2582
AD  - Department of Biomedical Engineering, Texas A&M University , College Station, 
      Texas 77843, USA.
FAU - Hinsdale, T
AU  - Hinsdale T
AD  - Department of Biomedical Engineering, Texas A&M University , College Station, 
      Texas 77843, USA.
FAU - Maitland, K
AU  - Maitland K
AUID- ORCID: 0000-0003-0931-0622
FAU - Cote, G
AU  - Cote G
LA  - eng
GR  - P30 ES023512/ES/NIEHS NIH HHS/United States
GR  - R44 ES022303/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20170324
PL  - United States
TA  - Biomicrofluidics
JT  - Biomicrofluidics
JID - 101293825
PMC - PMC5367146
EDAT- 2017/04/14 06:00
MHDA- 2017/04/14 06:01
PMCR- 2018/03/24
CRDT- 2017/04/14 06:00
PHST- 2017/01/17 00:00 [received]
PHST- 2017/03/14 00:00 [accepted]
PHST- 2017/04/14 06:00 [entrez]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/04/14 06:01 [medline]
PHST- 2018/03/24 00:00 [pmc-release]
AID - 1.4979198 [pii]
AID - 014702BMF [pii]
AID - 10.1063/1.4979198 [doi]
PST - epublish
SO  - Biomicrofluidics. 2017 Mar 24;11(2):024109. doi: 10.1063/1.4979198. eCollection 
      2017 Mar.