PMID- 28405463
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220317
IS  - 2055-5660 (Print)
IS  - 2055-5660 (Electronic)
IS  - 2055-5660 (Linking)
VI  - 3
DP  - 2017
TI  - The use of oral fluids to monitor key pathogens in porcine respiratory disease 
      complex.
PG  - 7
LID - 10.1186/s40813-017-0055-4 [doi]
LID - 7
AB  - BACKGROUND: The usefulness of oral fluid (OF) sampling for surveillance of 
      infections in pig populations is already accepted but its value as a tool to 
      support investigations of porcine respiratory disease complex (PRDC) has been 
      less well studied. This study set out to describe detection patterns of porcine 
      reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 
      (PCV2), swine influenza virus type A (SIV) and Mycoplasma hyopneumoniae (M. hyo) 
      among farms showing differing severity of PRDC. The study included six 
      wean-to-finish pig batches from farms with historical occurrence of respiratory 
      disease. OF samples were collected from six pens every two weeks from the 5(th) 
      to the 21(st) week of age and tested by real time PCR for presence of PRRSV, SIV 
      and M. hyo and by quantitative real time PCR for PCV2. Data was evaluated 
      alongside clinical and post-mortem observations, mortality rate, slaughter 
      pathology, histopathology, and immunohistochemistry testing data for PCV2 antigen 
      where available. RESULTS: PRRSV and M. hyo were detectable in OF but with 
      inconsistency between pens at the same sampling time and within pens over 
      sequential sampling times. Detection of SIV in clinical and subclinical cases 
      showed good consistency between pens at the same sampling time point with 
      detection possible for periods of 2-4 weeks. Quantitative testing of OF for PCV2 
      indicated different patterns and levels of detection between farms unaffected or 
      affected by porcine circovirus diseases (PCVD). There was good correlation of PCR 
      results for multiple samples collected from the same pen but no associations were 
      found between prevalence of positive test results and pen location in the 
      building or sex of pigs. CONCLUSIONS: Detection patterns for PRRSV, SIV and M. 
      hyo supported the effectiveness of OF testing as an additional tool for 
      diagnostic investigation of PRDC but emphasised the importance of sampling from 
      multiple pens and on multiple occasions. Preliminary evidence supported the 
      measurement of PCV2 load in pooled OF as a tool for prediction of clinical or 
      subclinical PCVD at farm level.
FAU - Hernandez-Garcia, Juan
AU  - Hernandez-Garcia J
AUID- ORCID: 0000-0001-5932-9327
AD  - Department of Veterinary Medicine, University of Cambridge, Madingley Road, 
      CB30ES Cambridge, England, UK. ISNI: 0000000121885934. GRID: grid.5335.0
FAU - Robben, Nardy
AU  - Robben N
AD  - Thermo Fisher Scientific, Waltham, MA USA. ISNI: 0000 0001 2187 0556. GRID: 
      grid.418190.5
FAU - Magnee, Damien
AU  - Magnee D
AD  - Thermo Fisher Scientific, Waltham, MA USA. ISNI: 0000 0001 2187 0556. GRID: 
      grid.418190.5
FAU - Eley, Thomas
AU  - Eley T
AD  - Royal Veterinary College, University of London, London, England, UK. ISNI: 0000 
      0001 2161 2573. GRID: grid.4464.2
FAU - Dennis, Ian
AU  - Dennis I
AD  - BQP Ltd., Stradbroke, England, UK.
FAU - Kayes, Sara M
AU  - Kayes SM
AD  - SAC Consulting Veterinary, Scotland's Rural College (SRUC), Penicuik, Midlothian 
      Scotland, UK.
FAU - Thomson, Jill R
AU  - Thomson JR
AD  - SAC Consulting Veterinary, Scotland's Rural College (SRUC), Penicuik, Midlothian 
      Scotland, UK.
FAU - Tucker, Alexander W
AU  - Tucker AW
AD  - Department of Veterinary Medicine, University of Cambridge, Madingley Road, 
      CB30ES Cambridge, England, UK. ISNI: 0000000121885934. GRID: grid.5335.0
LA  - eng
GR  - BB/G019274/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
DEP - 20170405
PL  - England
TA  - Porcine Health Manag
JT  - Porcine health management
JID - 101684126
PMC - PMC5382517
OTO - NOTNLM
OT  - Mycoplasma hyopneumoniae
OT  - Oral fluids
OT  - PCV2
OT  - PRRSV
OT  - SIV
EDAT- 2017/04/14 06:00
MHDA- 2017/04/14 06:01
PMCR- 2017/04/05
CRDT- 2017/04/14 06:00
PHST- 2016/10/25 00:00 [received]
PHST- 2017/02/13 00:00 [accepted]
PHST- 2017/04/14 06:00 [entrez]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/04/14 06:01 [medline]
PHST- 2017/04/05 00:00 [pmc-release]
AID - 55 [pii]
AID - 10.1186/s40813-017-0055-4 [doi]
PST - epublish
SO  - Porcine Health Manag. 2017 Apr 5;3:7. doi: 10.1186/s40813-017-0055-4. eCollection 
      2017.