PMID- 28405735
OWN - NLM
STAT- MEDLINE
DCOM- 20180416
LR  - 20181113
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 66
IP  - 8
DP  - 2017 Aug
TI  - Curcumin inhibits epigen and amphiregulin upregulated by 
      2,4,6-trinitrochlorobenzene associated with attenuation of skin swelling.
PG  - 663-678
LID - 10.1007/s00011-017-1048-0 [doi]
AB  - OBJECTIVES: Contact dermatitis model involving repeated application of hapten is 
      used as a tool to assess dermatitis, as characterized by thickening. Involvement 
      of cell proliferation, elicited by repeated hapten-stimulation, in this swelling 
      has been unclear. Curcumin is reported to reduce inflammation. We examined 
      involvement of cell proliferation and the role of extracellular regulated kinase 
      (ERK) in 2,4,6-trinitrochlorobenzene (TNCB) challenge-induced ear swelling. We 
      also examined the effects of curcumin in this model. METHODS: Mice were 
      sensitized with TNCB to the abdominal skin. Then, they were challenged with TNCB 
      to the ear three times. The ERK activation inhibitor U0126 or curcumin was 
      applied 30 min before each TNCB challenge. RESULTS: TNCB challenge-induced 
      increased epidermal cell number and dermal thickening. Gene expressions of 
      epithelial mitogen (EPGN), amphiregulin (AREG) and heparin-binding-epidermal 
      growth factor (HB-EGF) were increased in the ears after the last TNCB challenge. 
      Ki-67 immunoreactivity was increased in the dermis in TNCB-challenged ears. 
      TNCB-induced swelling was inhibited by U0126 and curcumin. Curcumin also 
      attenuated TNCB-induced ERK phosphorylation and expression of EPGN and AREG 
      genes. CONCLUSION: Ear swelling induced by TNCB challenge might be mediated, in 
      part, by the EPGN- and AREG-ERK proliferation pathway and was inhibited by 
      curcumin.
FAU - Sakai, Hiroyasu
AU  - Sakai H
AD  - Division of Pharmacy Professional Development and Research, Department of 
      Analytical Pathophysiology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan. sakai@hoshi.ac.jp.
FAU - Sato, Ken
AU  - Sato K
AD  - Division of Pharmacy Professional Development and Research, Department of 
      Analytical Pathophysiology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Sato, Fumiaki
AU  - Sato F
AD  - Division of Pharmacy Professional Development and Research, Department of 
      Analytical Pathophysiology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Kai, Yuki
AU  - Kai Y
AD  - Division of Pharmacy Professional Development and Research, Department of 
      Analytical Pathophysiology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Mandokoro, Kazutaka
AU  - Mandokoro K
AD  - Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Matsumoto, Kenjiro
AU  - Matsumoto K
AD  - Division of Pathological Sciences, Department of Pharmacology and Experimental 
      Therapeutics, Kyoto Pharmaceutical University, Yamashina, Kyoto, 607-8414, Japan.
FAU - Kato, Shinichi
AU  - Kato S
AD  - Division of Pathological Sciences, Department of Pharmacology and Experimental 
      Therapeutics, Kyoto Pharmaceutical University, Yamashina, Kyoto, 607-8414, Japan.
FAU - Yumoto, Tetsuro
AU  - Yumoto T
AD  - Division of Pharmacy Professional Development and Research, Department of 
      Analytical Pathophysiology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Narita, Minoru
AU  - Narita M
AD  - Department of Pharmacology, School of Pharmacy, Hoshi University, 2-4-41 Ebara, 
      Shinagawa-ku, Tokyo, 142-8501, Japan.
FAU - Chiba, Yoshihiko
AU  - Chiba Y
AD  - Department of Physiology and Molecular Sciences, School of Pharmacy, Hoshi 
      University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan.
LA  - eng
PT  - Journal Article
DEP - 20170412
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Epgn protein, mouse)
RN  - 0 (Epigen)
RN  - 0 (Haptens)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - IT942ZTH98 (Curcumin)
RN  - Z4ZG7O5SZ9 (Picryl Chloride)
SB  - IM
MH  - Amphiregulin/*metabolism
MH  - Animals
MH  - Curcumin/*pharmacology
MH  - Cytokines/genetics
MH  - Dermatitis, Allergic Contact/*metabolism/pathology
MH  - Epigen/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Female
MH  - Haptens
MH  - Intercellular Signaling Peptides and Proteins/genetics
MH  - Ki-67 Antigen/genetics/metabolism
MH  - Mice, Inbred BALB C
MH  - Phosphorylation/drug effects
MH  - Picryl Chloride
MH  - Skin/drug effects/metabolism/pathology
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Contact dermatitis
OT  - Curcumin
OT  - Epidermal growth factor
OT  - Swelling
EDAT- 2017/04/14 06:00
MHDA- 2018/04/17 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/03/07 00:00 [received]
PHST- 2017/04/06 00:00 [accepted]
PHST- 2017/04/04 00:00 [revised]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2018/04/17 06:00 [medline]
PHST- 2017/04/14 06:00 [entrez]
AID - 10.1007/s00011-017-1048-0 [pii]
AID - 10.1007/s00011-017-1048-0 [doi]
PST - ppublish
SO  - Inflamm Res. 2017 Aug;66(8):663-678. doi: 10.1007/s00011-017-1048-0. Epub 2017 
      Apr 12.