PMID- 28406442
OWN - NLM
STAT- MEDLINE
DCOM- 20170426
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 4
DP  - 2017 Apr 13
TI  - Liposomal Encapsulation for Systemic Delivery of Propranolol via Transdermal 
      Iontophoresis Improves Bone Microarchitecture in Ovariectomized Rats.
LID - 10.3390/ijms18040822 [doi]
LID - 822
AB  - The stimulatory effects of liposomal propranolol (PRP) on proliferation and 
      differentiation of human osteoblastic cells suggested that the prepared 
      liposomes-encapsulated PRP exerts anabolic effects on bone in vivo. Iontophoresis 
      provides merits such as sustained release of drugs and circumvention of first 
      pass metabolism. This study further investigated and evaluated the 
      anti-osteoporotic effects of liposomal PRP in ovariectomized (OVX) rats via 
      iontophoresis. Rats subjected to OVX were administered with pure or liposomal PRP 
      via iontophoresis or subcutaneous injection twice a week for 12 weeks. Changes in 
      the microarchitecture at the proximal tibia and the fourth lumbar spine were 
      assessed between pure or liposomal PRP treated and non-treated groups using 
      micro-computed tomography. Administration of liposomal PRP at low dose (0.05 
      mg/kg) via iontophoresis over 2-fold elevated ratio between bone volume and total 
      tissue volume (BV/TV) in proximal tibia to 9.0% whereas treatment with liposomal 
      PRP at low and high (0.5 mg/kg) doses via subcutaneous injection resulted in 
      smaller increases in BV/TV. Significant improvement of BV/TV and bone mineral 
      density (BMD) was also found in the fourth lumbar spine when low-dose liposomal 
      PRP was iontophoretically administered. Iontophoretic low-dose liposomal PRP also 
      elevated trabecular numbers in tibia and trabecular thickness in spine. 
      Enhancement of bone microarchitecture volumes has highlighted that liposomal 
      formulation with transdermal iontophoresis is promising for PRP treatment at the 
      lower dose and with longer duration than its clinical therapeutic range and 
      duration to exhibit optimal effects against bone loss in vivo.
FAU - Teong, Benjamin
AU  - Teong B
AD  - Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung City 80708, 
      Taiwan. benzblackcat@yahoo.com.
FAU - Kuo, Shyh Ming
AU  - Kuo SM
AD  - Department of Biomedical Engineering, I-Shou University, Kaohsiung City 82445, 
      Taiwan. smkuo@isu.edu.tw.
FAU - Tsai, Wei-Hsin
AU  - Tsai WH
AD  - Department of Veterinary Medicine, National Chiayi University, Chiayi City 60054, 
      Taiwan. s1050155@mail.ncyu.edu.tw.
FAU - Ho, Mei-Ling
AU  - Ho ML
AD  - Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung City 80708, 
      Taiwan. homelin@kmu.edu.tw.
FAU - Chen, Chung-Hwan
AU  - Chen CH
AD  - Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung City 80708, 
      Taiwan. hwan@kmu.edu.tw.
AD  - Department of Orthopaedics, College of Medicine, Kaohsiung Medical University, 
      Kaohsiung City 80708, Taiwan. hwan@kmu.edu.tw.
AD  - Department of Orthopedics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 
      Medical University, Kaohsiung City 80145, Taiwan. hwan@kmu.edu.tw.
FAU - Huang, Han Hsiang
AU  - Huang HH
AD  - Department of Veterinary Medicine, National Chiayi University, Chiayi City 60054, 
      Taiwan. hhuang@mail.ncyu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20170413
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Liposomes)
RN  - 27YLU75U4W (Phosphorus)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - AYI8EX34EU (Creatinine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Administration, Cutaneous
MH  - Animals
MH  - Blood Urea Nitrogen
MH  - Bone Density/drug effects
MH  - Bone and Bones/diagnostic imaging/physiology
MH  - Calcium/blood
MH  - Cholesterol/blood
MH  - Creatinine/blood
MH  - Drug Administration Schedule
MH  - Female
MH  - Iontophoresis
MH  - Liposomes/*chemistry
MH  - Liver/drug effects/metabolism
MH  - Lumbar Vertebrae/diagnostic imaging/drug effects/physiology
MH  - Osteoporosis/prevention & control
MH  - Ovariectomy
MH  - Phosphorus/blood
MH  - Propranolol/*chemistry/pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tibia/diagnostic imaging/drug effects/physiology
MH  - X-Ray Microtomography
PMC - PMC5412406
OTO - NOTNLM
OT  - bone loss
OT  - iontophoresis
OT  - liposome
OT  - propranolol
COIS- All authors have no conflict of interest.
EDAT- 2017/04/14 06:00
MHDA- 2017/04/27 06:00
PMCR- 2017/04/01
CRDT- 2017/04/14 06:00
PHST- 2017/02/18 00:00 [received]
PHST- 2017/03/15 00:00 [revised]
PHST- 2017/03/31 00:00 [accepted]
PHST- 2017/04/14 06:00 [entrez]
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2017/04/27 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijms18040822 [pii]
AID - ijms-18-00822 [pii]
AID - 10.3390/ijms18040822 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Apr 13;18(4):822. doi: 10.3390/ijms18040822.