PMID- 28406704
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20200115
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 66
IP  - 4
DP  - 2017 Sep 22
TI  - Pro-inflammatory gene expression in adipose tissue of patients with 
      atherosclerosis.
PG  - 633-640
AB  - Inflammatory changes, both in the arterial wall and adipose tissue, play a 
      crucial role in the development of atherosclerosis. We measured the gene 
      expression of tumor necrosis factor-alpha (TNFalpha), monocyte chemoattractant 
      protein-1 (MCP-1), and interleukin 6 (IL-6) in adipose tissue (AT) of living 
      kidney donors (LKD) and patients with peripheral arterial disease (PAD). 
      Quantitative polymerase chain reaction (qPCR) and flow cytometry analyses were 
      performed in subcutaneous (SAT), visceral (VAT), and perivascular adipose tissue 
      (PVAT). Data of PAD patients showed significantly higher expression in VAT in all 
      three genes (TNFalpha 5-fold, p<0.05; MCP-1 3.6-fold, p<0.05; IL-6 18.8-fold, 
      p<0.001). The differences in PVAT and SAT were less significant. Total body 
      pro-inflammatory status was documented by higher TNFalpha concentration in 
      patients (4.86+/-1.4 pg/ml) compared to LKDs (2.14+/-0.9 pg/ml; p<0.001), as was 
      hsCRP (11.8+/-7.0 in PAD; 1.5+/-0.48 in LKDs; p=0.017). We found no age-dependent 
      relationship between gene expression vs. TNFalpha and hsCRP concentrations in 
      both compared groups. No effect of the atherosclerosis score on gene expression 
      and circulating inflammatory markers within the PAD group was observed. Our 
      results suggest that the AT of PAD patients infiltrated with macrophages produces 
      more cytokines involved in the development of inflammation and atherosclerosis.
FAU - Cejkova, S
AU  - Cejkova S
AD  - Institute for Clinical and Experimental Medicine (IKEM), Prague, Czech Republic. 
      rupo@ikem.cz.
FAU - Kralova Lesna, I
AU  - Kralova Lesna I
FAU - Fronek, J
AU  - Fronek J
FAU - Janousek, L
AU  - Janousek L
FAU - Kralova, A
AU  - Kralova A
FAU - Zdychova, J
AU  - Zdychova J
FAU - Poledne, R
AU  - Poledne R
LA  - eng
PT  - Journal Article
DEP - 20170412
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (IL6 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adipose Tissue/*metabolism/pathology
MH  - Adult
MH  - Atherosclerosis/*genetics/*metabolism/pathology
MH  - Biomarkers/metabolism
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Inflammation Mediators/*metabolism
MH  - Interleukin-6/biosynthesis/genetics
MH  - Living Donors
MH  - Male
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 2017/04/14 06:00
MHDA- 2018/06/05 06:00
CRDT- 2017/04/14 06:00
PHST- 2017/04/14 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2017/04/14 06:00 [entrez]
AID - 933352 [pii]
AID - 10.33549/physiolres.933352 [doi]
PST - ppublish
SO  - Physiol Res. 2017 Sep 22;66(4):633-640. doi: 10.33549/physiolres.933352. Epub 
      2017 Apr 12.