PMID- 28407970
OWN - NLM
STAT- MEDLINE
DCOM- 20170512
LR  - 20220410
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 230
DP  - 2017 Sep 1
TI  - A simple and rapid method of isolation of active polypeptide complex, yolkin, 
      from chicken egg yolk.
PG  - 705-711
LID - S0308-8146(17)30482-X [pii]
LID - 10.1016/j.foodchem.2017.03.101 [doi]
AB  - A large number of bioactive peptides isolated from natural sources are known to 
      play important physiological roles in the human body. It is possible to use these 
      as alternative therapy agents. One example is yolkin which can be useful as a 
      food supplement, a natural therapeutic agent for preventing and treating 
      cognitive disorders of various origins, preferably in patients with 
      unsatisfactory responses to known therapies. A new simple method of isolation of 
      yolkin based on precipitation with ethanol or acetone was developed. The best 
      precipitation efficiency of both ethanol and acetone was achieved when stirred 
      into the starting material to a final concentration of 70%. These methods 
      preserved the ability of yolkin to stimulate human whole blood cells to release 
      anti-inflammatory cytokines and neurotrophins. At first we indicated that yolkin 
      displayed a potential neuroprotective effect by the ability to stimulate cells to 
      produce pro-survival brain derived neurotrophic factor (BDNF).
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Zambrowicz, A
AU  - Zambrowicz A
AD  - Department of Animal Products Technology and Quality Management, Faculty of Food 
      Sciences, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 
      37, 51-630 Wroclaw, Poland. Electronic address: 
      aleksandra.zambrowicz@upwr.edu.pl.
FAU - Zablocka, A
AU  - Zablocka A
AD  - Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of 
      Sciences, Immunochemistry, R. Weigla 12, 53-114 Wroclaw, Poland.
FAU - Bobak, L
AU  - Bobak L
AD  - Department of Animal Products Technology and Quality Management, Faculty of Food 
      Sciences, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 
      37, 51-630 Wroclaw, Poland.
FAU - Macala, J
AU  - Macala J
AD  - Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of 
      Sciences, Immunochemistry, R. Weigla 12, 53-114 Wroclaw, Poland.
FAU - Janusz, M
AU  - Janusz M
AD  - Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of 
      Sciences, Immunochemistry, R. Weigla 12, 53-114 Wroclaw, Poland.
FAU - Polanowski, A
AU  - Polanowski A
AD  - Department of Animal Products Technology and Quality Management, Faculty of Food 
      Sciences, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 
      37, 51-630 Wroclaw, Poland.
FAU - Trziszka, T
AU  - Trziszka T
AD  - Department of Animal Products Technology and Quality Management, Faculty of Food 
      Sciences, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 
      37, 51-630 Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170318
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Peptides)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*chemistry
MH  - Chickens
MH  - Egg Yolk/*chemistry
MH  - Humans
MH  - Peptides/*chemistry
OTO - NOTNLM
OT  - BDNF
OT  - Human whole blood cells
OT  - IL-10
OT  - IL-6
OT  - Polypeptide complex
OT  - Yolkin
EDAT- 2017/04/15 06:00
MHDA- 2017/05/13 06:00
CRDT- 2017/04/15 06:00
PHST- 2016/10/12 00:00 [received]
PHST- 2017/03/10 00:00 [revised]
PHST- 2017/03/17 00:00 [accepted]
PHST- 2017/04/15 06:00 [entrez]
PHST- 2017/04/15 06:00 [pubmed]
PHST- 2017/05/13 06:00 [medline]
AID - S0308-8146(17)30482-X [pii]
AID - 10.1016/j.foodchem.2017.03.101 [doi]
PST - ppublish
SO  - Food Chem. 2017 Sep 1;230:705-711. doi: 10.1016/j.foodchem.2017.03.101. Epub 2017 
      Mar 18.