PMID- 28408901
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 8
DP  - 2017
TI  - Serine-Rich Repeat Adhesins Contribute to Streptococcus gordonii-Induced 
      Maturation of Human Dendritic Cells.
PG  - 523
LID - 10.3389/fmicb.2017.00523 [doi]
LID - 523
AB  - Dendritic cells (DCs) play a pivotal role in the induction of immunity by 
      recognition, capture, process, and presentation of antigens from infectious 
      microbes. Streptococcus gordonii is able to cause life-threatening systemic 
      diseases such as infective endocarditis. Serine-rich repeat (SRR) glycoproteins 
      of S. gordonii are sialic acid-binding adhesins mediating the bacterial adherence 
      to the host and the development of infective endocarditis. Thus, the SRR adhesins 
      are potentially involved in the bacterial adherence to DCs and the maturation and 
      activation of DCs required for the induction of immunity to S. gordonii. Here, we 
      investigated the phenotypic and functional changes of human monocyte-derived DCs 
      treated with wild-type S. gordonii or the SRR adhesin-deficient mutant. The 
      mutant poorly bound to DCs and only weakly increased the expression of CD83, 
      CD86, MHC class II, and PD-L1 on DCs compared with the wild-type. In addition, 
      the mutant induced lower levels of TNF-alpha, IL-6, and IL-12 than the wild-type in 
      DCs. When DCs sensitized with the mutant were co-cultured with autologous T 
      cells, they induced weaker proliferation and activation of T cells than DCs 
      stimulated with the wild-type. Blockade of SRR adhesin with 3'-sialyllactose 
      markedly reduced S. gordonii binding and internalization, causing attenuation of 
      the bacterial immunostimulatory potency in DC maturation. Collectively, our 
      results suggest that SRR adhesins of S. gordonii are important for maturation and 
      activation of DCs.
FAU - Ko, Eun Byeol
AU  - Ko EB
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National UniversitySeoul, South Korea.
FAU - Kim, Sun Kyung
AU  - Kim SK
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National UniversitySeoul, South Korea.
FAU - Seo, Ho Seong
AU  - Seo HS
AD  - Biotechnology Research Division, Korea Atomic Energy Research InstituteJeongeup, 
      South Korea.
FAU - Yun, Cheol-Heui
AU  - Yun CH
AD  - Department of Agricultural Biotechnology and Research Institute for Agriculture 
      and Life Sciences, Seoul National UniversitySeoul, South Korea.
FAU - Han, Seung Hyun
AU  - Han SH
AD  - Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, 
      School of Dentistry, Seoul National UniversitySeoul, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20170331
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC5374164
OTO - NOTNLM
OT  - Streptococcus gordonii
OT  - T cell activation
OT  - dendritic cells
OT  - maturation
OT  - serine-rich repeat adhesins
EDAT- 2017/04/15 06:00
MHDA- 2017/04/15 06:01
PMCR- 2017/03/31
CRDT- 2017/04/15 06:00
PHST- 2016/09/21 00:00 [received]
PHST- 2017/03/13 00:00 [accepted]
PHST- 2017/04/15 06:00 [entrez]
PHST- 2017/04/15 06:00 [pubmed]
PHST- 2017/04/15 06:01 [medline]
PHST- 2017/03/31 00:00 [pmc-release]
AID - 10.3389/fmicb.2017.00523 [doi]
PST - epublish
SO  - Front Microbiol. 2017 Mar 31;8:523. doi: 10.3389/fmicb.2017.00523. eCollection 
      2017.